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http://purl.uniprot.org/citations/22511964http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22511964http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22511964http://www.w3.org/2000/01/rdf-schema#comment"

Background

The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds.

Methodology

The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gene was determined by high-density pyrosequencing and a genomic comparative analysis with other bla(NDM-1)-negative IncFII was performed.

Principal findings

Plasmid pGUE-NDM replicating in Escherichia coli confers resistance to many antibiotic molecules including β-lactams, aminoglycosides, trimethoprim, and sulfonamides. It is 87,022 bp in-size and carries the two β-lactamase genes bla(NDM-1) and bla(OXA-1), together with three aminoglycoside resistance genes aacA4, aadA2, and aacC2. Comparative analysis of the multidrug resistance locus contained a module encompassing the bla(NDM-1) gene that is actually conserved among different structures identified in other enterobacterial isolates. This module was constituted by the bla(NDM-1) gene, a fragment of insertion sequence ISAba125 and a bleomycin resistance encoding gene.

Significance

This is the first characterized bla(NDM-1)-carrying IncFII-type plasmid. Such association between the bla(NDM-1) gene and an IncFII-type plasmid backbone is extremely worrisome considering that this plasmid type is known to spread efficiently, as examplified with the worldwide dissemination of bla(CTX-M-15)-borne IncFII plasmids."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0034752"xsd:string
http://purl.uniprot.org/citations/22511964http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0034752"xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/author"Carattoli A."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/author"Carattoli A."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/author"Nordmann P."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/author"Nordmann P."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/author"Poirel L."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/author"Poirel L."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/author"Bonnin R.A."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/author"Bonnin R.A."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/name"PLoS ONE"xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/name"PLoS One"xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/pages"E34752"xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/pages"e34752"xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/title"Characterization of an IncFII Plasmid Encoding NDM-1 from Escherichia coli ST131."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/title"Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131."xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/volume"7"xsd:string
http://purl.uniprot.org/citations/22511964http://purl.uniprot.org/core/volume"7"xsd:string
http://purl.uniprot.org/citations/22511964http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22511964
http://purl.uniprot.org/citations/22511964http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22511964
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