| http://purl.uniprot.org/citations/22514732 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22514732 | http://www.w3.org/2000/01/rdf-schema#comment | "Calumenin isoforms 1 and 2 (calu-1/2), encoded by the CALU gene, belong to the CREC protein family. Calu-1/2 proteins are secreted into the extracellular space, but the secretory process and regulatory mechanism are largely unknown. Here, using a time-lapse imaging system, we visualized the intracellular transport and secretory process of calu-1/2-EGFP after their translocation into the ER lumen. Interestingly, we observed that an abundance of calu-1/2-EGFP accumulated in cellular processes before being released into the extracellular space, while only part of calu-1/2-EGFP proteins were secreted directly after attaching to the cell periphery. Moreover, we found the secretion of calu-1/2-EGFP required microtubule integrity, and that calu-1/2-EGFP-containing vesicles were transported by the motor proteins Kif5b and cytoplasmic dynein. Finally, we determined the export signal of calu-1/2-EGFP (amino acid positions 20-46) and provided evidence that the asparagine at site 131 was indispensable for calu-1/2-EGFP stabilization. Taken together, we provide a detailed picture of the intracellular transport of calu-1/2-EGFP, which facilitates our understanding of the secretory mechanism of calu-1/2."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0035344"xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Feng H."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Liu L."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Hao Q."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Shen B."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Wang Q."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Wang S."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Zheng P."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/author | "Teng J."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/pages | "e35344"xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/title | "The intracellular transport and secretion of calumenin-1/2 in living cells."xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/22514732 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22514732 |
| http://purl.uniprot.org/citations/22514732 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22514732 |
| http://purl.uniprot.org/uniprot/#_A8K048-mappedCitation-22514732 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22514732 |
| http://purl.uniprot.org/uniprot/#_B4DS27-mappedCitation-22514732 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22514732 |
| http://purl.uniprot.org/uniprot/#_Q2TA90-mappedCitation-22514732 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22514732 |
| http://purl.uniprot.org/uniprot/#_P33176-mappedCitation-22514732 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22514732 |