| http://purl.uniprot.org/citations/22528658 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22528658 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22528658 | http://www.w3.org/2000/01/rdf-schema#comment | "Interleukin-5 (IL-5), a major hematopoietin, stimulates eosinophil proliferation, migration, and activation, which have been implicated in the pathogenesis of allergic inflammatory diseases, such as asthma. The specific IL-5 receptor (IL-5R) consists of the IL-5 receptor α subunit (IL-5RA) and the common receptor β subunit (βc). IL-5 binding to IL-5R on target cells induces rapid tyrosine phosphorylation and activation of various cellular proteins, including JAK1/JAK2 and STAT1/STAT5. Here, we report the crystal structure of dimeric IL-5 in complex with the IL-5RA extracellular domains. The structure revealed that IL-5RA sandwiches the IL-5 homodimer by three tandem domains, arranged in a "wrench-like" architecture. This association mode was confirmed for human cells expressing IL-5 and the full-length IL-5RA by applying expanded genetic code technology: protein photo-cross-linking experiments revealed that the two proteins interact with each other in vivo in the same manner as that in the crystal structure. Furthermore, a comparison with the previously reported, partial GM-CSF•GM-CSFRA•βc structure enabled us to propose complete structural models for the IL-5 and GM-CSF receptor complexes, and to identify the residues conferring the cytokine-specificities of IL-5RA and GM-CSFRA."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.org/dc/terms/identifier | "doi:10.1002/pro.2072"xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.org/dc/terms/identifier | "doi:10.1002/pro.2072"xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Kusano S."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Kusano S."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Shirouzu M."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Shirouzu M."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Sakamoto K."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Sakamoto K."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Ohsawa N."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Ohsawa N."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Takatsu K."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Takatsu K."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Hino N."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Hino N."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Kukimoto-Niino M."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Kukimoto-Niino M."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Takaki S."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Takaki S."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Hara-Yokoyama M."xsd:string |
| http://purl.uniprot.org/citations/22528658 | http://purl.uniprot.org/core/author | "Hara-Yokoyama M."xsd:string |