| http://purl.uniprot.org/citations/22535961 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22535961 | http://www.w3.org/2000/01/rdf-schema#comment | "Certain Vibrio cholerae strains produce cholix, a potent protein toxin that has diphthamide-specific ADP-ribosyltransferase activity against eukaryotic elongation factor 2. Here we present a 1.8 Å crystal structure of cholix in complex with its natural substrate, nicotinamide adenine dinucleotide (NAD(+)). We also substituted hallmark catalytic residues by site-directed mutagenesis and analyzed both NAD(+) binding and ADP-ribosyltransferase activity using a fluorescence-based assay. These data are the basis for a new kinetic model of cholix toxin activity. Further, the new structural data serve as a reference for continuing inhibitor development for this toxin class."xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.337311"xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/author | "Jorgensen R."xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/author | "Fieldhouse R.J."xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/author | "Merrill A.R."xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/author | "Lugo M.R."xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/pages | "21176-21188"xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/title | "The 1.8 A cholix toxin crystal structure in complex with NAD+ and evidence for a new kinetic model."xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://purl.uniprot.org/core/volume | "287"xsd:string |
| http://purl.uniprot.org/citations/22535961 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22535961 |
| http://purl.uniprot.org/citations/22535961 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22535961 |
| http://purl.uniprot.org/uniprot/#_Q5EK40-mappedCitation-22535961 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22535961 |
| http://purl.uniprot.org/uniprot/Q5EK40 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22535961 |