| http://purl.uniprot.org/citations/22541168 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22541168 | http://www.w3.org/2000/01/rdf-schema#comment | "A highly sensitive hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method was developed and validated for the quantification of glycerophosphoinositol (GroPIns), glycerophosphocholine (GroPCho), glycerol 3-phosphate (GroP), inositol, and choline in the extracellular medium of Saccharomyces cerevisiae. The media samples were pretreated with a single two-phase liquid extraction. Chromatographic separation was achieved on a Waters Xbridge HILIC (150 mm × 4.6 mm, 5 μm) column under isocratic conditions using a mobile phase composed of acetonitrile/water, 70:30 (v/v) with 10mM ammonium acetate (pH adjusted to 4.5) at a flow-rate of 0.5 mL/min. Using a triple quadrupole tandem mass spectrometer, samples were detected in multiple reaction monitoring (MRM) mode via an electrospray ionization (ESI) source. The calibration curves were linear (r² ≥ 0.995) over the range of 0.5-150 nM, with the lower limit of quantitation validated at 0.5 nM for all analytes. The intra- and inter-day precision (calculated by coefficient of variation, CV%) ranged from 1.24 to 5.88% and 2.46 to 9.77%, respectively, and intra- and inter-day accuracy (calculated by relative error, RE%) was between -8.42 to 8.22% and -9.35 to 6.62%, respectively, at all quality control levels. The extracellular metabolites were stable throughout various storage stability studies. The fully validated method was successfully applied to determine the extracellular levels of phospholipid-related metabolites in S. cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jchromb.2012.03.034"xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/author | "Sun T."xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/author | "Patton-Vogt J."xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/author | "Johnson M.E."xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/author | "Surlow B.A."xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/author | "Wetzel S.J."xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/name | "J Chromatogr B Analyt Technol Biomed Life Sci"xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/pages | "1-9"xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/title | "Development and validation of a hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the quantification of lipid-related extracellular metabolites in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/22541168 | http://purl.uniprot.org/core/volume | "897"xsd:string |
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