| http://purl.uniprot.org/citations/22544940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22544940 | http://www.w3.org/2000/01/rdf-schema#comment | "CD4(+) T cell help contributes critically to DC-induced CD8(+) CTL immunity. However, precisely how these three cell populations interact and how CD4(+) T cell signals are delivered to CD8(+) T cells in vivo have been unclear. In this study, we developed a novel, two-step approach, wherein CD4(+) T cells and antigen-presenting DCs productively engaged one another in vivo in the absence of cognate CD8(+) T cells, after which, we selectively depleted the previously engaged CD4(+) T cells or DCs before allowing interactions of either population alone with naïve CD8(+) T cells. This protocol thus allows us to clearly document the importance of CD4(+) T-licensed DCs and DC-primed CD4(+) T cells in CTL immunity. Here, we provide direct in vivo evidence that primed CD4(+) T cells or licensed DCs can stimulate CTL response and memory, independent of DC-CD4(+) T cell clusters. Our results suggest that primed CD4(+) T cells with acquired pMHC-I from DCs represent crucial "immune intermediates" for rapid induction of CTL responses and for functional memory via CD40L signaling. Importantly, intravital, two-photon microscopy elegantly provide unequivocal in vivo evidence for direct CD4-CD8(+) T cell interactions via pMHC-I engagement. This study corroborates the coexistence of direct and indirect mechanisms of T cell help for a CTL response in noninflammatory situations. These data suggest a new "dynamic model of three-cell interactions" for CTL immunity derived from stimulation by dissociated, licensed DCs, primed CD4(+) T cells, and DC-CD4(+) T cell clusters and may have significant implications for autoimmunity and vaccine design."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.org/dc/terms/identifier | "doi:10.1189/jlb.1211631"xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/author | "Xiang J."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/author | "Mulligan S."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/author | "Gordon J.R."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/author | "Griebel P."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/author | "Ahmed K.A."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/author | "Munegowda M.A."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/author | "Mulligan S.J."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/name | "J Leukoc Biol"xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/pages | "289-300"xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/title | "Direct in vivo evidence of CD4+ T cell requirement for CTL response and memory via pMHC-I targeting and CD40L signaling."xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://purl.uniprot.org/core/volume | "92"xsd:string |
| http://purl.uniprot.org/citations/22544940 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22544940 |
| http://purl.uniprot.org/citations/22544940 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22544940 |
| http://purl.uniprot.org/uniprot/#_P01731-mappedCitation-22544940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22544940 |
| http://purl.uniprot.org/uniprot/#_Q8CAX3-mappedCitation-22544940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22544940 |
| http://purl.uniprot.org/uniprot/#_P27548-mappedCitation-22544940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22544940 |
| http://purl.uniprot.org/uniprot/#_Q0VEI3-mappedCitation-22544940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22544940 |
| http://purl.uniprot.org/uniprot/#_Q3UUV7-mappedCitation-22544940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22544940 |
| http://purl.uniprot.org/uniprot/#_Q542K6-mappedCitation-22544940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22544940 |
| http://purl.uniprot.org/uniprot/#_Q60965-mappedCitation-22544940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22544940 |