| http://purl.uniprot.org/citations/22578863 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22578863 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22578863 | http://www.w3.org/2000/01/rdf-schema#comment | "GTP-binding proteins (G-proteins) are highly conserved signaling molecules that participate in cellular signaling and bacterial pathogenesis by regulating the activity of cognate GTPases. However, the exact role of G-proteins in the pathogenesis of Mycobacterium tuberculosis is poorly understood. The complete genome sequence of M. tuberculosis H(37)Rv, suggests the presence of several homologs of bacterial G-proteins. In the present study, three G-proteins, Era, Obg and LepA of M. tuberculosis H(37)Rv were cloned and expressed in Escherichia coli. Purified proteins showed GTP-binding and hydrolyzing activities. A point mutation in the conserved GTP-binding motif, AspXXGly (Asp to Ala) in Era (Asp-258) and Obg (Asp-212) proteins resulted in the loss of the associated activities, confirming that known key residues in well-established G-proteins are also conserved in mycobacterial homologs. This study confirms that Era, Obg and LepA of M. tuberculosis H(37)Rv possess GTPase activity and provide a platform to understand the physiological significance of these proteins in associated pathogenesis."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.enzmictec.2007.08.008"xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.enzmictec.2007.08.008"xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/author | "Singh Y."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/author | "Singh Y."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/author | "Chopra P."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/author | "Chopra P."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/author | "Bedwal R.S."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/author | "Bedwal R.S."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/author | "Meena L.S."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/author | "Meena L.S."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/name | "Enzyme Microb. Technol."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/name | "Enzyme Microb. Technol."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/pages | "138-144"xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/pages | "138-144"xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/title | "Cloning and characterization of GTP-binding proteins of Mycobacterium tuberculosis H37Rv."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/title | "Cloning and characterization of GTP-binding proteins of Mycobacterium tuberculosis H37Rv."xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/volume | "42"xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://purl.uniprot.org/core/volume | "42"xsd:string |
| http://purl.uniprot.org/citations/22578863 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22578863 |
| http://purl.uniprot.org/citations/22578863 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22578863 |