| http://purl.uniprot.org/citations/22589305 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22589305 | http://www.w3.org/2000/01/rdf-schema#comment | "The SNF1/AMP-activated protein kinases are αβγ-heterotrimers that sense and regulate energy status in eukaryotes. They are activated by phosphorylation of the catalytic Snf1/α subunit, and the Snf4/γ regulatory subunit regulates phosphorylation through adenine nucleotide binding. In Saccharomyces cerevisiae, the Snf1 subunit is phosphorylated on the activation-loop Thr-210 in response to glucose limitation. To assess the requirement of the heterotrimer for regulated Thr-210 phosphorylation, we examined Snf1 and a truncated Snf1 kinase domain (residues 1-309) that has partial Snf1 function. Snf1(1-309) does not interact with the β and Snf4/γ regulatory subunits, and its activity was independent of them in vivo. Phosphorylation of both Snf1 and Snf1(1-309) increased in response to glucose limitation in wild-type cells and in cells lacking β- and Snf4/γ-subunits. These results indicate that glucose regulation of activation-loop phosphorylation can occur by mechanism(s) that function independently of the regulatory subunits. We further show that the Reg1-Glc7 protein phosphatase 1 and Sit4 type 2A-like phosphatase are largely responsible for dephosphorylation of Thr-210 of Snf1(1-309). Together, these findings suggest that these two phosphatases mediate heterotrimer-independent regulation of Thr-210 phosphorylation."xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1206280109"xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/author | "Xu X."xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/author | "Carlson M."xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/author | "Ruiz A."xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/pages | "8652-8657"xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/title | "Heterotrimer-independent regulation of activation-loop phosphorylation of Snf1 protein kinase involves two protein phosphatases."xsd:string |
| http://purl.uniprot.org/citations/22589305 | http://purl.uniprot.org/core/volume | "109"xsd:string |
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