| http://purl.uniprot.org/citations/22590603 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22590603 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22590603 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22590603 | http://www.w3.org/2000/01/rdf-schema#comment | "In this work, we report the complete genome sequence of an obligate aceticlastic methanogen, Methanosaeta harundinacea 6Ac. Genome comparison indicated that the three cultured Methanosaeta spp., M. thermophila, M. concilii and M. harundinacea 6Ac, each carry an entire suite of genes encoding the proteins involved in the methyl-group oxidation pathway, a pathway whose function is not well documented in the obligately aceticlastic methanogens. Phylogenetic analysis showed that the methyl-group oxidation-involving proteins, Fwd, Mtd, Mch, and Mer from Methanosaeta strains cluster with the methylotrophic methanogens, and were not closely related to those from the hydrogenotrophic methanogens. Quantitative PCR detected the expression of all genes for this pathway, albeit ten times lower than the genes for aceticlastic methanogenesis in strain 6Ac. Western blots also revealed the expression of fwd and mch, genes involved in methyl-group oxidation. Moreover, (13)C-labeling experiments suggested that the Methanosaeta strains might use the pathway as a methyl oxidation shunt during the aceticlastic metabolism. Because the mch mutants of Methanosarcina barkeri or M. acetivorans failed to grow on acetate, we suggest that Methanosaeta may use methyl-group oxidation pathway to generate reducing equivalents, possibly for biomass synthesis. An fpo operon, which encodes an electron transport complex for the reduction of CoM-CoB heterodisulfide, was found in the three genomes of the Methanosaeta strains. However, an incomplete protein complex lacking the FpoF subunit was predicted, as the gene for this protein was absent. Thus, F(420)H(2) was predicted not to serve as the electron donor. In addition, two gene clusters encoding the two types of heterodisulfide reductase (Hdr), hdrABC, and hdrED, respectively, were found in the three Methanosaeta genomes. Quantitative PCR determined that the expression of hdrED was about ten times higher than hdrABC, suggesting that hdrED plays a major role in aceticlastic methanogenesis."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0036756"xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0036756"xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0036756"xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Dong X."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Dong X."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Dong X."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Liu D."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Liu D."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Liu D."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zhang G."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zhang G."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zhang G."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zhu J."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zhu J."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zhu J."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zheng H."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zheng H."xsd:string |
| http://purl.uniprot.org/citations/22590603 | http://purl.uniprot.org/core/author | "Zheng H."xsd:string |