| http://purl.uniprot.org/citations/22634595 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22634595 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22634595 | http://www.w3.org/2000/01/rdf-schema#comment | "Apically enriched Rab11-positive recycling endosomes (Rab11-REs) are important for establishing and maintaining epithelial polarity. Yet, little is known about the molecules controlling trafficking of Rab11-REs in an epithelium in vivo. Here, we report a genome-wide, image-based RNA interference screen for regulators of Rab11-RE positioning and transport of an apical membrane protein (PEPT-1) in C. elegans intestine. Among the 356 screen hits was the 14-3-3 and partitioning defective protein PAR-5, which we found to be specifically required for Rab11-RE positioning and apicobasal polarity maintenance. Depletion of PAR-5 induced abnormal clustering of Rab11-REs to ectopic sites at the basolateral cortex containing F-actin and other apical domain components. This phenotype required key regulators of F-actin dynamics and polarity, such as Rho GTPases (RHO-1 and the Rac1 orthologue CED-10) and apical PAR proteins. Our data suggest that PAR-5 acts as a regulatory hub for a polarity-maintaining network required for apicobasal asymmetry of F-actin and proper Rab11-RE positioning."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncb2508"xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncb2508"xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Habermann B."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Habermann B."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Zerial M."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Zerial M."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Marsico G."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Marsico G."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Hoepfner S."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Hoepfner S."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Bradshaw C.R."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Bradshaw C.R."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Farnung B.O."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Farnung B.O."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Korn K."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Korn K."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Volkmer M."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Volkmer M."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Winter J.F."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/author | "Winter J.F."xsd:string |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22634595 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |