| http://purl.uniprot.org/citations/22638108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22638108 | http://www.w3.org/2000/01/rdf-schema#comment | "The lymphovascular embolus is an enigmatic entity adept at metastatic dissemination and chemotherapy resistance. Using MARY-X, a human breast cancer xenograft that exhibits florid lymphovascular emboli in mice and spheroids in vitro, we established a model where the in vitro transition stages from minced tumoral aggregates to well-formed spheroids served as a surrogate for in vivo emboli formation. MARY-X well-formed spheroids and emboli exhibited strong similarity of expression. The aggregate-to-spheroid transition stages were characterized by increased ExoC5, decreased Hgs and Rab7, increased calpains, increased full-length E-cadherin (E-cad/FL), and the transient appearance of E-cad/NTF2, a 95 kDa E-cadherin fragment and increased Notch3icd (N3icd), the latter two fragments produced by increased γ-secretase. Both transient and permanent knockdowns of Rab7 in MCF-7 cells increased protein but not transcription of E-cad/FL and resulted in the de novo appearance of E-cad/NTF2, the presence of nuclear E-cad/CTF2, and increased Notch1icd (N1icd). Overexpression of Rab7 conversely decreased E-cad/FL, γ-secretase (PS1/NTF), and E-cad/NTF2. Overexpression of calpains did not alter PS1/NTF but decreased E-cad/FL and E-cad/NTF2 and increased N1icd. Well-formed spheroids showed increased Rab7, absent E-cad/NTF2, decreased PS1/NTF, increased E-cad/NTF1, and increased N3icd, the latter two fragments being the direct and indirect consequences, respectively, of increased calpains (calpain 1 and calpain 2). Inhibition of calpains decreased E-cad/NTF1 but increased E-cad/NTF2 showing that calpains compete with γ-secretase (PS1) for closely located cleavage/binding sites on E-cadherin and that increased calpains can shuttle even decreased levels of γ-secretase to Notch 3, resulting in increased Notch 3 signaling in the well-formed spheroids."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.org/dc/terms/identifier | "doi:10.1158/1541-7786.mcr-12-0009"xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/author | "Tian H."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/author | "Ye Y."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/author | "Barsky S.H."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/author | "Lange A.R."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/author | "Robertson F.M."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/author | "Yearsley K."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/author | "Gao J.X."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/name | "Mol Cancer Res"xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/pages | "713-726"xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/title | "Early to intermediate steps of tumor embolic formation involve specific proteolytic processing of E-cadherin regulated by Rab7."xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/22638108 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22638108 |
| http://purl.uniprot.org/citations/22638108 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22638108 |
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| http://purl.uniprot.org/uniprot/#_A0A140HMB1-mappedCitation-22638108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22638108 |
| http://purl.uniprot.org/uniprot/#_A0A158RFU6-mappedCitation-22638108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22638108 |
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| http://purl.uniprot.org/uniprot/#_B3GN61-mappedCitation-22638108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22638108 |