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| http://purl.uniprot.org/citations/22639648 | http://www.w3.org/2000/01/rdf-schema#comment | "Arabidopsis miR319a/b/c primary transcripts are unusual due to the presence of a long stem and loop structure containing functional miR319a/b/c molecules. In our experiments carried out using high throughput sequencing (HTS), we have shown that additional microRNAs (miRNAs), miR319a.2/b.2/c.2 are generated from the upper part of the same hairpin structure. We have also found cognate miRNAa.2*/b.2*/c.2* to be present in the HTS results with a considerably lower number of reads. Northern hybridization revealed that miR319b.2 is mainly expressed in 35-day-old plant rosette leaves, as well as in stem and inflorescences of 42- and 53-day-old plants. Moreover, it carries multiple signatures of a functional miRNA, including as follows: (i) its biogenesis is HYL1-dependent; (ii) it is incorporated in a substantial amount into RISC complexes containing AGO1, AGO2, or AGO4 protein; (iii) 24 nt-long species of miR319b.2 have been found in inflorescences to be more abundant than 21 nt miR319b.2 species; (iv) it is present in various ratios to miR319b during plant development, which suggests the existence of a regulatory mechanism responsible for its biogenesis/processing; (v) there is an observed cross-species conservation of the miR319a/b/c stem nucleotide sequence extending beyond mature miRNA region; and (vi) all evidence suggests that intron-containing RAP2.12 mRNA isoform is the target for miR319b.2. All these features prompt us to claim miR319b.2 as a functional miRNA molecule."xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.org/dc/terms/identifier | "doi:10.3389/fpls.2012.00046"xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/author | "Jarmolowski A."xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/author | "Szweykowska-Kulinska Z."xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/author | "Sobkowiak L."xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/author | "Karlowski W."xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/name | "Front Plant Sci"xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/pages | "46"xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/title | "Non-Canonical Processing of Arabidopsis pri-miR319a/b/c Generates Additional microRNAs to Target One RAP2.12 mRNA Isoform."xsd:string |
| http://purl.uniprot.org/citations/22639648 | http://purl.uniprot.org/core/volume | "3"xsd:string |
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