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http://purl.uniprot.org/citations/22645333http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22645333http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22645333http://www.w3.org/2000/01/rdf-schema#comment"Movement of the plant hormone abscisic acid (ABA) within plants has been documented; however, the molecular mechanisms that regulate ABA transport are not fully understood. By using a modified yeast two-hybrid system, we screened Arabidopsis cDNAs capable of inducing interactions between the ABA receptor PYR/PYL/RCAR and PP2C protein phosphatase under low ABA concentrations. By using this approach, we identified four members of the NRT1/PTR family as candidates for ABA importers. Transport assays in yeast and insect cells demonstrated that at least one of the candidates ABA-IMPORTING TRANSPORTER (AIT) 1, which had been characterized as the low-affinity nitrate transporter NRT1.2, mediates cellular ABA uptake. Compared with WT, the ait1/nrt1.2 mutants were less sensitive to exogenously applied ABA during seed germination and/or postgermination growth, whereas overexpression of AIT1/NRT1.2 resulted in ABA hypersensitivity in the same conditions. Interestingly, the inflorescence stems of ait1/nrt1.2 had a lower surface temperature than those of the WT because of excess water loss from open stomata. We detected promoter activities of AIT1/NRT1.2 around vascular tissues in inflorescence stems, leaves, and roots. These data suggest that the function of AIT1/NRT1.2 as an ABA importer at the site of ABA biosynthesis is important for the regulation of stomatal aperture in inflorescence stems."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.org/dc/terms/identifier"doi:10.1073/pnas.1203567109"xsd:string
http://purl.uniprot.org/citations/22645333http://purl.org/dc/terms/identifier"doi:10.1073/pnas.1203567109"xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Chiba Y."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Chiba Y."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Koshiba T."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Koshiba T."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Kamiya Y."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Kamiya Y."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Ichikawa T."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Ichikawa T."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Matsui M."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Matsui M."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Nakazawa M."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Nakazawa M."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Hanada A."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Hanada A."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Seo M."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Seo M."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Kanno Y."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/author"Kanno Y."xsd:string
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22645333http://purl.uniprot.org/core/date"2012"xsd:gYear