| http://purl.uniprot.org/citations/22699487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22699487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22699487 | http://www.w3.org/2000/01/rdf-schema#comment | "Epididymal protease inhibitor (EPPIN) is found on the surface of spermatozoa and works as a central hub for a sperm surface protein complex (EPPIN protein complex [EPC]) that inhibits sperm motility on the binding of semenogelin I (SEMG1) during ejaculation. Here, we identify EPPIN's amino acids involved in the interactions within the EPC and demonstrate that EPPIN's sequence C102-P133 contains the major binding site for SEMG1. Within the same region, the sequence F117-P133 binds the EPC-associated protein lactotransferrin (LTF). We show that residues Cys102, Tyr107, and Phe117 in the EPPIN C-terminus are required for SEMG1 binding. Additionally, residues Tyr107 and Phe117 are critically involved in the interaction between EPPIN and LTF. Our findings demonstrate that EPPIN is a key player in the protein-protein interactions within the EPC. Target identification is an important step toward the development of a novel male contraceptive, and the functionality of EPPIN's residues Cys102, Tyr107, and Phe117 offers novel opportunities for contraceptive compounds that inhibit sperm motility by targeting this region of the molecule."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.org/dc/terms/identifier | "doi:10.1095/biolreprod.112.101832"xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.org/dc/terms/identifier | "doi:10.1095/biolreprod.112.101832"xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/author | "O'Rand M.G."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/author | "O'Rand M.G."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/author | "Richardson R.T."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/author | "Richardson R.T."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/author | "Hamil K.G."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/author | "Hamil K.G."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/author | "Silva E.J."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/author | "Silva E.J."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/name | "Biol. Reprod."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/name | "Biol. Reprod."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/pages | "56"xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/pages | "56"xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/title | "Characterization of EPPIN's semenogelin I binding Site: a contraceptive drug target."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/title | "Characterization of EPPIN's semenogelin I binding Site: a contraceptive drug target."xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/volume | "87"xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://purl.uniprot.org/core/volume | "87"xsd:string |
| http://purl.uniprot.org/citations/22699487 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22699487 |
| http://purl.uniprot.org/citations/22699487 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22699487 |