| http://purl.uniprot.org/citations/22705946 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22705946 | http://www.w3.org/2000/01/rdf-schema#comment | "Regulated exocytosis requires that the assembly of the basic membrane fusion machinery is temporarily arrested. Synchronized membrane fusion is then caused by a specific trigger--a local rise of the Ca(2+) concentration. Using reconstituted giant unilamellar vesicles (GUVs), we have analysed the role of complexin and membrane-anchored synaptotagmin 1 in arresting and synchronizing fusion by lipid-mixing and cryo-electron microscopy. We find that they mediate the formation and consumption of docked small unilamellar vesicles (SUVs) via the following sequence of events: Synaptotagmin 1 mediates v-SNARE-SUV docking to t-SNARE-GUVs in a Ca(2+)-independent manner. Complexin blocks vesicle consumption, causing accumulation of docked vesicles. Together with synaptotagmin 1, complexin synchronizes and stimulates rapid fusion of accumulated docked vesicles in response to physiological Ca(2+) concentrations. Thus, the reconstituted assay resolves both the stimulatory and inhibitory function of complexin and mimics key aspects of synaptic vesicle fusion."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2012.164"xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/author | "Briggs J.A."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/author | "Bharat T.A."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/author | "Sollner T.H."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/author | "Malsam J."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/author | "Krause J.M."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/author | "Parisotto D."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/author | "Scheutzow A."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/pages | "3270-3281"xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/title | "Complexin arrests a pool of docked vesicles for fast Ca2+-dependent release."xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/22705946 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22705946 |
| http://purl.uniprot.org/citations/22705946 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22705946 |
| http://purl.uniprot.org/uniprot/#_O14810-mappedCitation-22705946 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22705946 |
| http://purl.uniprot.org/uniprot/#_P21579-mappedCitation-22705946 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22705946 |
| http://purl.uniprot.org/uniprot/#_J3KQA0-mappedCitation-22705946 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22705946 |
| http://purl.uniprot.org/uniprot/J3KQA0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22705946 |
| http://purl.uniprot.org/uniprot/P21579 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22705946 |
| http://purl.uniprot.org/uniprot/O14810 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22705946 |