http://purl.uniprot.org/citations/22733760 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/22733760 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/22733760 | http://www.w3.org/2000/01/rdf-schema#comment | "REPRESSOR OF SILENCING 1 (ROS1) is a DNA demethylation enzyme that was previously identified during a genetic screen for the silencing of both RD29A-LUC and 35S-NPTII transgenes on a T-DNA construct. Here we performed a genetic screen to identify additional mutants in which the 35S-NPTII transgene is silenced. We identified several alleles of ros1 and of the following components of the RNA-directed DNA methylation (RdDM) pathway: NRPD1 (the largest subunit of polymerase IV), RDR2, NRPE1 (the largest subunit of polymerase V), NRPD2, AGO4, and DMS3. Our results show that the silencing of 35S-NPTII in the RdDM pathway mutants is due to the reduced expression of ROS1 in the mutants. We also identified a putative histone acetyltransferase (ROS4) from the genetic screen. The acetyltransferase contains a PHD-finger domain that binds to unmethylated histone H3K4. The mutation in ROS4 led to reduction of H3K18 and H3K23 acetylation levels. We show that the silencing of 35S-NPTII and some transposable element genes was released by the ddm1 mutation but that this also required ROS4. Our study identifies a unique antisilencing factor, and reveals that the RdDM pathway has an antisilencing function due to its role in maintaining ROS1 expression."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1208557109"xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1208557109"xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Gong Z."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Gong Z."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Qian W."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Qian W."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Shen J."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Shen J."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Wang C."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Wang C."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Zhao Y."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Zhao Y."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Zhu J.K."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/author | "Zhu J.K."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/pages | "11425-11430"xsd:string |
http://purl.uniprot.org/citations/22733760 | http://purl.uniprot.org/core/pages | "11425-11430"xsd:string |