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http://purl.uniprot.org/citations/22737924http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22737924http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To study the expression of lung Krüppel-like transcription factor (KLF2/LKLF) in lung tissues of rats with chronic obstructive pulmonary disease (COPD) and the relationship between KLF2 and NF-E2-related factor 2 (Nrf2), and make further explore the effects of KLF2 on the expression of gamma-glutamylcysteine synthetase (gamma-GCS).

Methods

Twenty-two male SD rats were randomly divided into a COPD group (n = 10) and a normal control group (n = 11). The rat model of COPD established by cigarette smoking and intratracheal instillation of lipopolysaccharide (LPS), and lung tissues were obtained. The expressions of KLF2, Nrf2, gamma-GCS mRNA and protein in lung tissues were measured by immunohistochemistry (IHC), Western blot, in situ hybridization (ISH) and reverse transcription-polymerase chain reaction (RT-PCR). To explore the relationship between KLF2 and Nrf2 protein,we utilize the method of co-immunoprecipitation (CO-IP).

Results

IHC and Western blot showed that protein expressions of KLF2, Nrf2, gamma-GCS were higher in the lung tissues from rats with COPD than those in the control groups (all P < 0.05). The levels of KLF2, gamma-GCS mRNA were markedly increased in the COPD group (all P < 0.01) while Nrf2 mRNA expression in COPD group had no significant difference with that in control group ( P > 0.05). CO-IP result showed that KLF2 were obviously present in immunoprecipitates of Nrf2 (P < 0.01) . Linear correlation analysis showed that the level of KLF2 protein was positively correlated with the level of Nrf2 protein (P < 0.05), and KLF2, Nrf2 proteins were positively correlated with gamma-GCS mRNA and protein (all P < 0.05).

Conclusion

The expression of KLF2 is significantly up-regulated in COPD, which maybe up-regulate gamma-GCS mRNA expression by increasing Nrf2 expression and nuclear translocation against oxidative stress."xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/author"Liu X.Y."xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/author"Tan S.X."xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/author"Dai A.G."xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/author"Hu R.C."xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/author"Zhu L.M."xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/name"Zhongguo Ying Yong Sheng Li Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/pages"173-178"xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/title"[The effect of transcription factor KLF2 in expression of gamma-GCS depend on regulation by Nrf2 in lung of rats with chronic obstructive pulmonary disease]."xsd:string
http://purl.uniprot.org/citations/22737924http://purl.uniprot.org/core/volume"28"xsd:string
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