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http://purl.uniprot.org/citations/22766194http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22766194http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22766194http://www.w3.org/2000/01/rdf-schema#comment"

Background

CD10, also known as neprilysin or enkephalinase exhibiting neutral endopeptidase (NEP) activity, is expressed by B-lineage hematopoietic cells as well as a variety of cells from normal tissues. It cleaves peptides such as cytokines to act for terminating inflammatory responses. Although CD10 molecules of the human pre-B-cell line NALM-6 have 6 consensus N-glycosylation sites, three of them are known to be N-glycosylated by X-ray crystallography.

Methods

In order to investigate the role of N-glycans in the full expression of NEP activity, we modified N-glycans by treatment of NALM6 cells with various glycosidases or alter each of the consensus N-glycosylation sites by generating site-directed mutagenesis and compared the NEP activities of the sugar-altered CD10 with those of intact CD10.

Results

CD10 of the human B-cell line NALM-6 was dominantly localized in raft microdomains and heterogeneously N-glycosylated. Although neither desialylation nor further degalactosylation caused defective NEP activity, removal of only a small part of N-glycans by treatment with glycopeptidase F under non-denaturing conditions decreased NEP activity completely. All of the three consensus sites of CD10 in HEK293 cells introduced with wild type-CD10 were confirmed to be N-glycosylated. Surface expression of N-glycan at Asn(628)-deleted CD10 by HEK293 cells was greatly decreased as well as it lost entire NEP activities.

Conclusions

N-glycosylation at Asn(628) is essential not only for NEP activities, but also for surface expression.

General significance

Quality control system does not allow dysfunctional ecto-type proteases to express on plasma membrane."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.org/dc/terms/identifier"doi:10.1016/j.bbagen.2012.06.017"xsd:string
http://purl.uniprot.org/citations/22766194http://purl.org/dc/terms/identifier"doi:10.1016/j.bbagen.2012.06.017"xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Ito S."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Ito S."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Yamada H."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Yamada H."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Iijima K."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Iijima K."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Fujimoto J."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Fujimoto J."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Sato B."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Sato B."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Katagiri Y.U."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Katagiri Y.U."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Kiyokawa N."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Kiyokawa N."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Okita H."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Okita H."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Kawasaki N."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/author"Kawasaki N."xsd:string
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22766194http://purl.uniprot.org/core/date"2012"xsd:gYear
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