| http://purl.uniprot.org/citations/22768349 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22768349 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22768349 | http://www.w3.org/2000/01/rdf-schema#comment | "In the Caenorhabditis elegans nematode, the oocyte nucleolus disappears prior to fertilization. We have now investigated the re-formation of the nucleolus in the early embryo of this model organism by immunostaining for fibrillarin and DAO-5, a putative NOLC1/Nopp140 homolog involved in ribosome assembly. We find that labeled nucleoli first appear in somatic cells at around the 8-cell stage, at a time when transcription of the embryonic genome begins. Quantitative analysis of radial positioning showed the nucleolus to be localized at the nuclear periphery in a majority of early embryonic nuclei. At the ultrastructural level, the embryonic nucleolus appears to be composed of a relatively homogenous core surrounded by a crescent-shaped granular structure. Prior to embryonic genome activation, fibrillarin and DAO-5 staining is seen in numerous small nucleoplasmic foci. This staining pattern persists in the germline up to the ∼100-cell stage, until the P4 germ cell divides to give rise to the Z2/Z3 primordial germ cells and embryonic transcription is activated in this lineage. In the ncl-1 mutant, which is characterized by increased transcription of rDNA, DAO-5-labeled nucleoli are already present at the 2-cell stage. Our results suggest a link between the activation of transcription and the initial formation of nucleoli in the C. elegans embryo."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0040290"xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0040290"xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Lanctot C."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Lanctot C."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Raska I."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Raska I."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Cmarko D."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Cmarko D."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Gombitova A."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Gombitova A."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Korcekova D."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/author | "Korcekova D."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/pages | "E40290"xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/pages | "E40290"xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/title | "Nucleologenesis in the Caenorhabditis elegans embryo."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/title | "Nucleologenesis in the Caenorhabditis elegans embryo."xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/22768349 | http://purl.uniprot.org/core/volume | "7"xsd:string |