| http://purl.uniprot.org/citations/22771797 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22771797 | http://www.w3.org/2000/01/rdf-schema#comment | "The transmembrane protease complex γ-secretase is responsible for the generation of the neurotoxic amyloid β-peptide (Aβ) from its precursor (APP). Aβ has a causative role in Alzheimer disease, and thus, γ-secretase is a therapeutic target. However, since there are more than 70 γ-secretase substrates besides APP, selective inhibition of APP processing is required. Recent data indicates the existence of several γ-secretase associated proteins (GSAPs) that affect the selection and processing of substrates. Here, we use a γ-secretase inhibitor for affinity purification of γ-secretase and associated proteins from microsomes and detergent resistant membranes (DRMs) prepared from rat or human brain. By tandem mass spectrometry we identified a novel brain GSAP; erlin-2. This protein was recently reported to reside in DRMs in the ER. A proximity ligation assay, as well as co-immunoprecipitation, confirmed the association of erlin-2 with γ-secretase. We found that a higher proportion of erlin-2 was associated with γ-secretase in DRMs than in soluble membranes. siRNA experiments indicated that reduced levels of erlin-2 resulted in a decreased Aβ production, whereas the effect on Notch processing was limited. In summary, we have found a novel brain GSAP, erlin-2, that resides in DRMs and affects Aβ production."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2012.06.137"xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Ishikawa T."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Nishimura T."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Winblad B."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Teranishi Y."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Kihara T."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Tjernberg L.O."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Hur J.Y."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Gu G.J."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Kamali-Moghaddam M."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Frykman S."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/author | "Behbahani H."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/pages | "476-481"xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/title | "Erlin-2 is associated with active gamma-secretase in brain and affects amyloid beta-peptide production."xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://purl.uniprot.org/core/volume | "424"xsd:string |
| http://purl.uniprot.org/citations/22771797 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22771797 |
| http://purl.uniprot.org/citations/22771797 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22771797 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GQZ0-mappedCitation-22771797 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22771797 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GRG7-mappedCitation-22771797 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22771797 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GRQ1-mappedCitation-22771797 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22771797 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GRS4-mappedCitation-22771797 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22771797 |