| http://purl.uniprot.org/citations/22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22773836 | http://www.w3.org/2000/01/rdf-schema#comment | "MARCKS is an actin-binding protein that modulates vascular endothelial cell migration and cytoskeleton signaling (Kalwa, H., and Michel, T. (2011) J. Biol. Chem. 286, 2320-2330). Angiotensin-II is a vasoactive peptide implicated in vascular physiology as well as pathophysiology; the pathways connecting angiotensin-II and cytoskeletal remodeling are incompletely understood. Here we show that MARCKS is expressed in intact arterial preparations, with prominent staining of the endothelium. In endothelial cells, angiotensin-II-promoted MARCKS phosphorylation is abrogated by PEG-catalase, implicating endogenous H(2)O(2) in the angiotensin-II response. Studies using the H(2)O(2) biosensor HyPer2 reveal that angiotensin-II promotes increases in intracellular H(2)O(2). We used a Rac1 FRET biosensor to show that angiotensin-II promotes Rac1 activation that is attenuated by PEG-catalase. siRNA-mediated Rac1 knockdown blocks angiotensin-II-stimulated MARCKS phosphorylation. Cell imaging studies using a phosphoinositide 4,5-bisphosphate (PIP(2)) biosensor revealed that angiotensin-II PIP(2) regulation depends on MARCKS and H(2)O(2). siRNA-mediated knockdown of MARCKS or Rac1 attenuates receptor-mediated activation of the tyrosine kinase c-Abl and disrupts actin fiber formation. These studies establish a critical role for H(2)O(2) in angiotensin-II signaling to the endothelial cytoskeleton in a novel pathway that is critically dependent on MARCKS, Rac1, and c-Abl."xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m112.381517"xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/author | "Kalwa H."xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/author | "Michel T."xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/author | "Sartoretto J.L."xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/author | "Sartoretto S.M."xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/pages | "29147-29158"xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/title | "Angiotensin-II and MARCKS: a hydrogen peroxide- and RAC1-dependent signaling pathway in vascular endothelium."xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://purl.uniprot.org/core/volume | "287"xsd:string |
| http://purl.uniprot.org/citations/22773836 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22773836 |
| http://purl.uniprot.org/citations/22773836 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22773836 |
| http://purl.uniprot.org/uniprot/#_A0A0A1HAM8-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_Q05C82-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_Q8BPG5-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_K7Q7T7-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_Q05BL6-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_P11859-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_P26645-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_P29966-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_P63001-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_Q3TLP8-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |
| http://purl.uniprot.org/uniprot/#_Q3UTR7-mappedCitation-22773836 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22773836 |