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http://purl.uniprot.org/citations/22777005http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22777005http://www.w3.org/2000/01/rdf-schema#comment"

Rationale

Endothelial adherens junction proteins constitute an important element in the control of microvascular permeability. Platelet-activating factor (PAF) increases permeability to macromolecules via translocation of endothelial nitric oxide synthase (eNOS) to cytosol and stimulation of eNOS-derived nitric oxide signaling cascade. The mechanisms by which nitric oxide signaling regulates permeability at adherens junctions are still incompletely understood.

Objective

We explored the hypothesis that PAF stimulates hyperpermeability via S-nitrosation (SNO) of adherens junction proteins.

Methods and results

We measured PAF-stimulated SNO of β-catenin and p120-catenin (p120) in 3 cell lines: ECV-eNOSGFP, EAhy926 (derived from human umbilical vein), and postcapillary venular endothelial cells (derived from bovine heart endothelium) and in the mouse cremaster muscle in vivo. SNO correlated with diminished abundance of β-catenin and p120 at the adherens junction and with hyperpermeability. Tumor necrosis factor-α increased nitric oxide production and caused similar increase in SNO as PAF. To ascertain the importance of eNOS subcellular location in this process, we used ECV-304 cells transfected with cytosolic eNOS (GFPeNOSG2A) and plasma membrane eNOS (GFPeNOSCAAX). PAF induced SNO of β-catenin and p120 and significantly diminished association between these proteins in cells with cytosolic eNOS but not in cells wherein eNOS is anchored to the cell membrane. Inhibitors of nitric oxide production and of SNO blocked PAF-induced SNO and hyperpermeability, whereas inhibition of the cGMP pathway had no effect. Mass spectrometry analysis of purified p120 identified cysteine 579 as the main S-nitrosated residue in the region that putatively interacts with vascular endothelial-cadherin.

Conclusions

Our results demonstrate that agonist-induced SNO contributes to junctional membrane protein changes that enhance endothelial permeability."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.org/dc/terms/identifier"doi:10.1161/circresaha.112.274548"xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Gonzalez F.G."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Zamorano P."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Marin N."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Carrasco R."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Quezada C."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Meininger C.J."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Duran W.N."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Boric M.P."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Sanchez F.A."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/author"Mujica P."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/name"Circ Res"xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/pages"553-563"xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/title"S-Nitrosation of beta-catenin and p120 catenin: a novel regulatory mechanism in endothelial hyperpermeability."xsd:string
http://purl.uniprot.org/citations/22777005http://purl.uniprot.org/core/volume"111"xsd:string
http://purl.uniprot.org/citations/22777005http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22777005
http://purl.uniprot.org/citations/22777005http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/22777005
http://purl.uniprot.org/uniprot/#_E9Q8Z5-mappedCitation-22777005http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22777005
http://purl.uniprot.org/uniprot/#_E9Q8Z6-mappedCitation-22777005http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22777005
http://purl.uniprot.org/uniprot/#_E9Q8Z8-mappedCitation-22777005http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22777005
http://purl.uniprot.org/uniprot/#_E9Q905-mappedCitation-22777005http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22777005
http://purl.uniprot.org/uniprot/#_E9Q986-mappedCitation-22777005http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22777005
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