| http://purl.uniprot.org/citations/22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22778260 | http://www.w3.org/2000/01/rdf-schema#comment | "Gephyrin is a scaffold protein essential for the postsynaptic clustering of inhibitory glycine and different subtypes of GABA(A) receptors. The cellular and molecular mechanisms involved in gephyrin-mediated receptor clustering are still not well understood. Here we provide evidence that the gephyrin-binding protein collybistin is involved in regulating the phosphorylation of gephyrin. We demonstrate that the widely used monoclonal antibody mAb7a is a phospho-specific antibody that allows the cellular and biochemical analysis of gephyrin phosphorylation at Ser-270. In addition, another neighbored epitope determinant was identified at position Thr-276. Analysis of the double mutant gephyrin(T276A,S277A) revealed significant reduction in gephyrin cluster formation and altered oligomerization behavior of gephyrin. Moreover, pharmacological inhibition of cyclin-dependent kinases in hippocampal neurons reduced postsynaptic gephyrin mAb7a immunoreactivities. In vitro phosphorylation assays and phosphopeptide competition experiments revealed a phosphorylation at Ser-270 depending on enzyme activities of cyclin-dependent kinases CDK1, -2, or -5. These data indicate that collybistin and cyclin-dependent kinases are involved in regulating the phosphorylation of gephyrin at postsynaptic membrane specializations."xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m112.349597"xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/author | "Schlicksupp A."xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/author | "Kirsch J."xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/author | "Nawrotzki R."xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/author | "Kuhse J."xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/author | "Mukusch S."xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/author | "Kalbouneh H."xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/pages | "30952-30966"xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/title | "Phosphorylation of gephyrin in hippocampal neurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin."xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://purl.uniprot.org/core/volume | "287"xsd:string |
| http://purl.uniprot.org/citations/22778260 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22778260 |
| http://purl.uniprot.org/citations/22778260 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22778260 |
| http://purl.uniprot.org/uniprot/#_A0A090N7W4-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z355-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |
| http://purl.uniprot.org/uniprot/#_A0A5F9ZHY9-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |
| http://purl.uniprot.org/uniprot/#_A0A2X0SYV8-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |
| http://purl.uniprot.org/uniprot/#_B2RDJ7-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |
| http://purl.uniprot.org/uniprot/#_B7Z2B5-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |
| http://purl.uniprot.org/uniprot/#_B7Z4J7-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |
| http://purl.uniprot.org/uniprot/#_B4DGQ8-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |
| http://purl.uniprot.org/uniprot/#_A6IQ19-mappedCitation-22778260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22778260 |