| http://purl.uniprot.org/citations/22789293 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22789293 | http://www.w3.org/2000/01/rdf-schema#comment | "Understanding protein and gene function requires identifying interaction partners using biochemical, molecular or genetic tools. In plants, searching for novel protein-protein interactions is limited to protein purification assays, heterologous in vivo systems such as the yeast-two-hybrid or mutant screens. Ideally one would be able to search for novel protein partners in living plant cells. We demonstrate that it is possible to screen for novel protein-protein interactions from a random library in protoplasted Arabidopsis plant cells and recover some of the interacting partners. Our screen is based on capturing the bi-molecular complementation of mYFP between an YN-bait fusion partner and a completely random prey YC-cDNA library with FACS. The candidate interactions were confirmed using in planta BiFC assays and in planta FRET-FLIM assays. From this work, we show that the well characterized protein Calcium Dependent Protein Kinase 3 (CPK3) interacts with APX3, HMGB5, ORP2A and a ricin B-related lectin domain containing protein At2g39050. This is one of the first randomin planta screens to be successfully employed."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.org/dc/terms/identifier | "doi:10.1186/1746-4811-8-25"xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Zhou Y."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Harter K."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Wallmeroth N."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Berendzen K.W."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Bohmer M."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Schleifenbaum F."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Peter S."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Tiesler F.K."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/author | "Vesic M."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/name | "Plant Methods"xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/pages | "25"xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/title | "Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry."xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/22789293 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22789293 |
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| http://purl.uniprot.org/uniprot/#_Q0WSD0-mappedCitation-22789293 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22789293 |
| http://purl.uniprot.org/uniprot/#_O49597-mappedCitation-22789293 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22789293 |
| http://purl.uniprot.org/uniprot/#_O82388-mappedCitation-22789293 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22789293 |
| http://purl.uniprot.org/uniprot/#_Q2V3V9-mappedCitation-22789293 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22789293 |