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http://purl.uniprot.org/citations/22804620http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22804620http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22804620http://www.w3.org/2000/01/rdf-schema#comment"

Background

In the Rh blood group system, variant RhD and RhCE express several partial antigens. We investigated RH in samples with partial DIVa that demonstrated weak and variable reactivity with anti-C.

Study design and methods

Standard hemagglutination techniques, polymerase chain reaction-based assays, and RH sequencing were used.

Results

DNA analysis showed that six red blood cell (RBC) samples with weak and inconsistent reactivity with anti-C lacked RHCE*C, but all had RHD*DIVa, which encodes partial D and Go(a) . We then tested RBCs from 19 Go(a+) cryopreserved samples (confirmed to have RHD*DIVa) with four anti-C and observed weak variable reactions. RHCE genotyping found all but one of the samples with RHD*DIVa also had RHCE nt 48G>C and 1025C>T, named RHCE*ceTI. Lookback of samples referred for workup and found to have either allele revealed 47 of 55 had both RHD*DIVa and RHCE*ceTI, four had RHD*DIVa without RHCE*ceTI, and four had RHCE*ceTI without RHD*DIVa. Alloanti-c was found in a patient with c+ RBCs and RHCE*ceTI in trans to RHCE*Ce, and alloanti-e was found in a patient with e+ RBC and RHCE*ceTI in trans to RHCE*cE. RHD*DIVa in trans to RHD erroneously tested as RHD hemizygous.

Conclusions

RHD*DIVa and RHCE*ceTI almost always, but not invariably, travel together. This haplotype is found in people of African ancestry and the RBCs can demonstrate aberrant reactivity with anti-C. RHCE*ceTI encodes partial c and e antigens. We confirm that RHD zygosity assays are unreliable in samples with RHD*DIVa."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.org/dc/terms/identifier"doi:10.1111/j.1537-2995.2012.03800.x"xsd:string
http://purl.uniprot.org/citations/22804620http://purl.org/dc/terms/identifier"doi:10.1111/j.1537-2995.2012.03800.x"xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Copeland T."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Copeland T."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Reid M.E."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Reid M.E."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Horn T."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Horn T."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Westhoff C.M."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Westhoff C.M."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Hue-Roye K."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Hue-Roye K."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Velliquette R.W."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Velliquette R.W."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Lomas-Francis C."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Lomas-Francis C."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Vege S."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Vege S."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Halter Hipsky C."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/author"Halter Hipsky C."xsd:string
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/22804620http://purl.uniprot.org/core/date"2013"xsd:gYear
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