| http://purl.uniprot.org/citations/22813754 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22813754 | http://www.w3.org/2000/01/rdf-schema#comment | "Cysteine desulfurases generate a covalent persulfide intermediate from cysteine, and this activated form of sulfur is essential for the synthesis of iron-sulfur (Fe-S) clusters. In yeast mitochondria, there is a complete machinery for Fe-S cluster synthesis, including a cysteine desulfurase, Nfs1p. Here we show that following supplementation of isolated mitochondria with [(35)S]cysteine, a radiolabeled persulfide could be detected on Nfs1p. The persulfide persisted under conditions that did not permit Fe-S cluster formation, such as nucleotide and/or iron depletion of mitochondria. By contrast, under permissive conditions, the radiolabeled Nfs1p persulfide was greatly reduced and radiolabeled aconitase was formed, indicating transfer of persulfide to downstream Fe-S cluster recipients. Nfs1p in mitochondria was found to be relatively more resistant to inactivation by N-ethylmaleimide (NEM) as compared with a prokaryotic cysteine desulfurase. Mitochondria treated with NEM (1 mM) formed the persulfide on Nfs1p but failed to generate Fe-S clusters on aconitase, likely due to inactivation of downstream recipient(s) of the Nfs1p persulfide. Thus the Nfs1p-bound persulfide as described here represents a precursor en route to Fe-S cluster synthesis in mitochondria."xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.mito.2012.07.103"xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/author | "Dancis A."xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/author | "Lyver E.R."xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/author | "Pandey A."xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/author | "Pain D."xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/author | "Yoon H."xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/name | "Mitochondrion"xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/pages | "539-549"xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/title | "Identification of a Nfs1p-bound persulfide intermediate in Fe-S cluster synthesis by intact mitochondria."xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/22813754 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22813754 |
| http://purl.uniprot.org/citations/22813754 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22813754 |
| http://purl.uniprot.org/uniprot/#_P25374-mappedCitation-22813754 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22813754 |
| http://purl.uniprot.org/uniprot/P25374 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22813754 |