| http://purl.uniprot.org/citations/22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22837015 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundKnowledge of gene expression kinetics around neuronal cell birth is required to dissect mechanisms underlying progenitor fate. Here, we timed cell cycle and neuronal protein silencing/induction during cell birth in the developing murine retina.ResultsThe pan-cell cycle markers Pcna and Mcm6 were present in the post-mitotic ganglion cell layer. Although confined to the neuroblastic layer (NBL), 6-7% of Ki67(+) cells lacked six progenitor/cell cycle markers, and expressed neuronal markers. To define protein extinction/induction timing, we defined G2/M length throughout retinogenesis, which was typically 1-2 h, but <10% cells took double this time. BrdU-chase analyses revealed that at E12.5, Tubb3 (Tuj1) appeared at M-phase, followed by Calb2 and Dcx at ~2 h, Elavl2/3/4 at ~4 h, and Map2 at ~6 h after cell birth, and these times extended with embryonic age. Strikingly, Ki67 was not extinguished until up to a day after cell cycle exit, coinciding with exit from the NBL and induction of late markers such as Map1b/Uchl1/Rbfox3.ConclusionsA minor population of progenitors transits slowly through G2/M and, most importantly, some cell cycle proteins are retained for an unexpectedly long period in post-mitotic neurons. The high-resolution map of cell birth kinetics reported here provides a framework to better define mechanisms that regulate neurogenesis."xsd:string |
| http://purl.uniprot.org/citations/22837015 | http://purl.org/dc/terms/identifier | "doi:10.1002/dvdy.23840"xsd:string |
| http://purl.uniprot.org/citations/22837015 | http://purl.uniprot.org/core/author | "Bremner R."xsd:string |
| http://purl.uniprot.org/citations/22837015 | http://purl.uniprot.org/core/author | "Pacal M."xsd:string |
| http://purl.uniprot.org/citations/22837015 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22837015 | http://purl.uniprot.org/core/name | "Dev Dyn"xsd:string |
| http://purl.uniprot.org/citations/22837015 | http://purl.uniprot.org/core/pages | "1525-1544"xsd:string |
| http://purl.uniprot.org/citations/22837015 | http://purl.uniprot.org/core/title | "Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post-mitotic newborn neurons."xsd:string |
| http://purl.uniprot.org/citations/22837015 | http://purl.uniprot.org/core/volume | "241"xsd:string |
| http://purl.uniprot.org/citations/22837015 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22837015 |
| http://purl.uniprot.org/citations/22837015 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22837015 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JEY1-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A0A1L1SS95-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A0A1L1SUG6-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GQV9-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A0A1D5RM76-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_P63015-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_Q1KS26-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A0A286YDV1-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A2A409-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A2A412-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A2A4W9-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A2A9R4-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |
| http://purl.uniprot.org/uniprot/#_A2A9R5-mappedCitation-22837015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22837015 |