| http://purl.uniprot.org/citations/22855528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22855528 | http://www.w3.org/2000/01/rdf-schema#comment | "The sialomucin endolyn is implicated in adhesion, migration, and differentiation of various cell types. Along rat kidney tubules, endolyn is variously localized to the apical surface and endosomal/lysosomal compartments. Apical delivery of newly synthesized rat endolyn predominates over direct lysosomal delivery in polarized Madin-Darby canine kidney cells. Apical sorting depends on terminal processing of a subset of lumenal N-glycans. Here we dissect the requirements of N-glycan processing for apical targeting and investigate the underlying mechanism. Modulation of glycan branching and subsequent polylactosamine elongation by knockdown of N-acetylglucosaminyltransferase III or V had no effect on apical delivery of endolyn. In contrast, combined but not individual knockdown of sialyltransferases ST3Gal-III, ST3Gal-IV, and ST6Gal-I, which together are responsible for addition of α2,3- and α2,6-linked sialic acids on N-glycans, dramatically decreased endolyn surface polarity. Endolyn synthesized in the presence of kifunensine, which blocks terminal N-glycan processing, reduced its interaction with several recombinant canine galectins, and knockdown of galectin-9 (but not galectin-3, -4, or -8) selectively disrupted endolyn polarity. Our data suggest that sialylation enables recognition of endolyn by galectin-9 to mediate efficient apical sorting. They raise the intriguing possibility that changes in glycosyltransferase expression patterns and/or galectin-9 distribution may acutely modulate endolyn trafficking in the kidney."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e12-04-0329"xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/author | "Youker R.T."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/author | "Hughey R.P."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/author | "Weisz O.A."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/author | "Mo D."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/author | "Ihrke G."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/author | "Costa S.A."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/author | "Pastor-Soler N."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/pages | "3636-3646"xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/title | "Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism."xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/22855528 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22855528 |
| http://purl.uniprot.org/citations/22855528 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22855528 |
| http://purl.uniprot.org/uniprot/#_A6HH78-mappedCitation-22855528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22855528 |
| http://purl.uniprot.org/uniprot/#_F7ETI8-mappedCitation-22855528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22855528 |
| http://purl.uniprot.org/uniprot/#_P97840-mappedCitation-22855528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22855528 |
| http://purl.uniprot.org/uniprot/A6HH78 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22855528 |
| http://purl.uniprot.org/uniprot/F7ETI8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22855528 |
| http://purl.uniprot.org/uniprot/P97840 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22855528 |