| http://purl.uniprot.org/citations/22875945 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22875945 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22875945 | http://www.w3.org/2000/01/rdf-schema#comment | "Acidification of synaptic vesicles relies on the vacuolar-type ATPase (V-ATPase) and provides the electrochemical driving force for neurotransmitter exchange. The regulatory mechanisms that ensure assembly of the V-ATPase holoenzyme on synaptic vesicles are unknown. Rabconnectin3α (Rbc3α) is a potential candidate for regulation of V-ATPase activity because of its association with synaptic vesicles and its requirement for acidification of intracellular compartments. Here, we provide the first evidence for a role of Rbc3α in synaptic vesicle acidification and neurotransmission. In this study, we characterized mutant alleles of rbc3α isolated from a large-scale screen for zebrafish with auditory/vestibular defects. We show that Rbc3α is localized to basal regions of hair cells in which synaptic vesicles are present. To determine whether Rbc3α regulates V-ATPase activity, we examined the acidification of synaptic vesicles and localization of the V-ATPase in hair cells. In contrast to wild-type hair cells, we observed that synaptic vesicles had elevated pH, and a cytosolic subunit of the V-ATPase was no longer enriched in synaptic regions of mutant hair cells. As a consequence of defective acidification of synaptic vesicles, afferent neurons in rbc3α mutants had reduced firing rates and reduced accuracy of phase-locked action potentials in response to mechanical stimulation of hair cells. Collectively, our data suggest that Rbc3α modulates synaptic transmission in hair cells by promoting V-ATPase activity in synaptic vesicles."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.1705-12.2012"xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/author | "Liu Q."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/author | "Liu Q."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/author | "Nicolson T."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/author | "Nicolson T."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/author | "Einhorn Z."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/author | "Einhorn Z."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/author | "Trapani J.G."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/author | "Trapani J.G."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/name | "J. Neurosci."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/name | "J Neurosci"xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/pages | "11144-11156"xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/pages | "11144-11156"xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/title | "Rabconnectin3alpha promotes stable activity of the H+ pump on synaptic vesicles in hair cells."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/title | "Rabconnectin3alpha promotes stable activity of the H+ pump on synaptic vesicles in hair cells."xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/22875945 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22875945 |
| http://purl.uniprot.org/citations/22875945 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22875945 |
| http://purl.uniprot.org/citations/22875945 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22875945 |