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http://purl.uniprot.org/citations/22879580http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22879580http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Thromboxane A(2) and prostacyclin are thromboregulatory prostaglandins. The inflammatory C-reactive protein (CRP) promotes thrombosis after vascular injury, presumably via potentiation of thromboxane activity. Using a genetic approach, we investigated the role of thromboxane receptor (TP) pathway in CRP-induced thrombosis.

Methods and results

Four genetically engineered mice strains were used: C57BL/6 wild-type, human CRP transgenic (CRPtg), thromboxane receptor-deficient (Tp(-/-)), and CRPtgTp(-/-) mice. CRP and TP expression were correlated, and suppression of CRP expression using small interfering RNA/CRP led to reduction in TP expression. Platelet-endothelial adherence was increased in CRPtg and suppressed in CRPtgTP(-/-)and CRPtg cells that were suppressed with TP small interfering RNA. TP deficiency in both platelets and endothelial cells was synergistic in affecting platelet-endothelial interactions. Time until arterial occlusion, measured after photochemical injury, was significantly shorter in CRPtg and prolonged in CRPtgTp(-/-) compared with controls (n=10-15, 35±3.4, 136±13.8, and 67±8.9 minutes, respectively; P<0.05).

Conclusions

TP pathway is of major importance in CRP-induced thrombosis. The expression of TP is increased in CRPtg endothelial cells, and its blockade significantly suppresses the prothrombotic effect of CRP."xsd:string
http://purl.uniprot.org/citations/22879580http://purl.org/dc/terms/identifier"doi:10.1161/atvbaha.112.256073"xsd:string
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/author"FitzGerald G.A."xsd:string
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/author"Danenberg H.D."xsd:string
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/author"Grad E."xsd:string
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/author"Pachino R.M."xsd:string
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/name"Arterioscler Thromb Vasc Biol"xsd:string
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/pages"2468-2474"xsd:string
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/title"Role of thromboxane receptor in C-reactive protein-induced thrombosis."xsd:string
http://purl.uniprot.org/citations/22879580http://purl.uniprot.org/core/volume"32"xsd:string
http://purl.uniprot.org/citations/22879580http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22879580
http://purl.uniprot.org/citations/22879580http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/22879580
http://purl.uniprot.org/uniprot/#_P30987-mappedCitation-22879580http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22879580
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http://purl.uniprot.org/uniprot/#_Q712P6-mappedCitation-22879580http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22879580
http://purl.uniprot.org/uniprot/#_Q5FW61-mappedCitation-22879580http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22879580
http://purl.uniprot.org/uniprot/Q712P5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22879580
http://purl.uniprot.org/uniprot/P30987http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22879580
http://purl.uniprot.org/uniprot/Q5FW61http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22879580
http://purl.uniprot.org/uniprot/Q712P6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22879580