| http://purl.uniprot.org/citations/22879814 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22879814 | http://www.w3.org/2000/01/rdf-schema#comment | "Eukaryotic circadian clocks rely on transcriptional feedback loops. In Drosophila, the PERIOD (PER) and TIMELESS (TIM) proteins accumulate during the night, inhibit the activity of the CLOCK (CLK)/CYCLE (CYC) transcriptional complex, and are degraded in the early morning. The control of PER and TIM oscillations largely depends on post-translational mechanisms. They involve both light-dependent and light-independent pathways that rely on the phosphorylation, ubiquitination, and proteasomal degradation of the clock proteins. SLMB, which is part of a CULLIN-1-based E3 ubiquitin ligase complex, is required for the circadian degradation of phosphorylated PER. We show here that CULLIN-3 (CUL-3) is required for the circadian control of PER and TIM oscillations. Expression of either Cul-3 RNAi or dominant negative forms of CUL-3 in the clock neurons alters locomotor behavior and dampens PER and TIM oscillations in light-dark cycles. In constant conditions, CUL-3 deregulation induces behavioral arrhythmicity and rapidly abolishes TIM cycling, with slower effects on PER. CUL-3 affects TIM accumulation more strongly in the absence of PER and forms protein complexes with hypo-phosphorylated TIM. In contrast, SLMB affects TIM more strongly in the presence of PER and preferentially associates with phosphorylated TIM. CUL-3 and SLMB show additive effects on TIM and PER, suggesting different roles for the two ubiquitination complexes on PER and TIM cycling. This work thus shows that CUL-3 is a new component of the Drosophila clock, which plays an important role in the control of TIM oscillations."xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pbio.1001367"xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/author | "Grima B."xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/author | "Lamouroux A."xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/author | "Rouyer F."xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/author | "Chelot E."xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/author | "Dognon A."xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/name | "PLoS Biol"xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/pages | "e1001367"xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/title | "CULLIN-3 controls TIMELESS oscillations in the Drosophila circadian clock."xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/22879814 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22879814 |
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