| http://purl.uniprot.org/citations/22889848 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22889848 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22889848 | http://www.w3.org/2000/01/rdf-schema#comment | "Two type I interferon (IFN) genes, designated as rbIFN1 and rbIFN2, have been cloned and characterized in rock bream. They are both comprised of 5 exons and 4 introns, and are closely linked on the rock bream chromosome in a unique head-to-head configuration. Both genes encode 183 amino acid (aa) precursor with a putative 17 aa signal peptide in the N-terminal. Only one amino acid divergence is present between two IFNs. Compared with the type I IFNs in higher vertebrates, two rock bream IFNs possess conserved alpha helical structure and share approximately 20% identity in aa sequence. The highest aa sequence homology (83.2%) was found with European seabass IFNs. Phylogenetic analysis grouped two rock bream IFNs into the subgroup-d of two-cysteine containing IFNs. The gene synteny analysis revealed that they are orthologous with the zebrafish IFNφ4 on chromosome-12 and paralogous to each other, which are likely derived from a gene duplication event followed by an inversion. A number of cis-regulatory elements associated with immune response including 15 IRF and 6 NF-κB binding sites are predicted in the shared 4.5 kb 5'-flanking region. Highest constitutive expression of two IFNs was detected in blood cells and skin. Their expression in blood cells and head kidney was up-regulated by lipopolysaccharide, poly I:C, Edwardsiella tarda, Streptococcus iniae and iridovirus. Furthermore, recombinant rbIFN1 protein produced by E. coli induced a rapid and transient expression of the interferon inducible Mx gene in head kidney cells. These results suggest that two duplicated type I IFN genes are involved in rock bream host response to both viral and bacterial pathogens."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.fsi.2012.07.018"xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.fsi.2012.07.018"xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Lee J."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Lee J."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Jung S.J."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Jung S.J."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Oh M.J."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Oh M.J."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Wan Q."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Wan Q."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Kim H.C."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Kim H.C."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Whang I."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Whang I."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Lim B.S."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Lim B.S."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Yeo S.Y."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Yeo S.Y."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Wicramaarachchi W.D."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/author | "Wicramaarachchi W.D."xsd:string |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22889848 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |