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http://purl.uniprot.org/citations/22921913http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22921913http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To study the regulation of apoptosis in human endometrial cells. The specific aims were to determine whether milk fat globule-epidermal growth factor 8 (MFG-E8), a novel endometrial epithelial protein, modulates caspase activation and DNA fragmentation; and to examine whether hCG, an early embryonic product, regulates Bax and Bcl-2 equilibrium, as well as MFG-E8 expression.

Design

Primary cultures of human endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs).

Setting

Academic center.

Patient(s)

Ovulatory women aged 21-30 years.

Intervention(s)

Treatment with MFG-E8 and hCG.

Main outcome measure(s)

Apoptotic activity was quantified using a luciferase assay. Deoxyribonucleic acid fragmentation was detected by TUNEL assay. Bax, Bcl-2, and MFG-E8 messenger RNA expression levels were determined by quantitative reverse transcription-polymerase chain reaction. Immunocytochemistry was used to establish cell purity and presence of MFG-E8 and hCG-R (receptor) proteins.

Result(s)

Endometrial epithelial cells were cytokeratin(+), vimentin(-), MFG-E8(+), and hCG-R(+), whereas ESC were vimentin(+), cytokeratin(-), MFG-E8(-), and hCG-R(+). Treatment of ESC with MFG-E8 resulted in a 13-fold increase in caspase activity and a 30-fold increase in TUNEL. On the other hand, hCG decreased messenger RNA expression of Bax in ESC.

Conclusion(s)

Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. Conversely, pregnancy levels of hCG has opposite effects on stromal cells."xsd:string
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http://purl.uniprot.org/citations/22921913http://purl.uniprot.org/core/author"Anderson S."xsd:string
http://purl.uniprot.org/citations/22921913http://purl.uniprot.org/core/author"Franchi A."xsd:string
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http://purl.uniprot.org/citations/22921913http://purl.uniprot.org/core/author"Riggs R.M."xsd:string
http://purl.uniprot.org/citations/22921913http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22921913http://purl.uniprot.org/core/name"Fertil Steril"xsd:string
http://purl.uniprot.org/citations/22921913http://purl.uniprot.org/core/pages"1549-56.e3"xsd:string
http://purl.uniprot.org/citations/22921913http://purl.uniprot.org/core/title"Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium."xsd:string
http://purl.uniprot.org/citations/22921913http://purl.uniprot.org/core/volume"98"xsd:string
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