| http://purl.uniprot.org/citations/22925462 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22925462 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22925462 | http://www.w3.org/2000/01/rdf-schema#comment | "Pathogenic mycobacteria require type VII secretion (T7S) systems to transport virulence factors across their complex cell envelope. These bacteria have up to five of these systems, termed ESX-1 to ESX-5. Here, we show that ESX-5 of Mycobacterium tuberculosis mediates the secretion of EsxN, PPE and PE_PGRS proteins, indicating that ESX-5 is a major secretion pathway in this important pathogen. Using the ESX-5 system of Mycobacterium marinum and Mycobacterium bovis BCG as a model, we have purified and analysed the T7S membrane complex under native conditions. blue native-PAGE and immunoprecipitation experiments showed that the ESX-5 membrane complex of both species has a size of ~ 1500 kDa and is composed of four conserved membrane proteins, i.e. EccB(5) , EccC(5) , EccD(5) and EccE(5) . Subsequent limited proteolysis suggests that EccC(5) and EccE(5) mostly reside on the periphery of the complex. We also observed that EccC(5) and EccD(5) expression is essential for the formation of a stable membrane complex. These are the first data on a T7S membrane complex and, given the high conservation of its components, our data can likely be generalized to most mycobacterial T7S systems."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.2012.08206.x"xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.2012.08206.x"xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Ottenhoff T.H."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Ottenhoff T.H."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Wilson L."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Wilson L."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Piersma S.R."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Piersma S.R."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Bitter W."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Bitter W."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Luirink J."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Luirink J."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Bestebroer J."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Bestebroer J."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Houben E.N."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Houben E.N."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Jimenez C.R."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Jimenez C.R."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Ummels R."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/author | "Ummels R."xsd:string |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22925462 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |