| http://purl.uniprot.org/citations/22940582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22940582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22940582 | http://www.w3.org/2000/01/rdf-schema#comment | "Proteins of unknown function comprise a significant fraction of sequenced genomes. Defining the roles of these proteins is vital to understanding cellular processes. Here, we describe a method to determine a protein function based on the identification of its natural ligand(s) by the crystallographic screening of the binding of a metabolite library, followed by a focused search in the metabolic space. The method was applied to two protein families with unknown function, PF01256 and YjeF_N. The PF01256 proteins, represented by YxkO from Bacillus subtilis and the C-terminal domain of Tm0922 from Thermotoga maritima, were shown to catalyze ADP/ATP-dependent NAD(P)H-hydrate dehydratation, a previously described orphan activity. The YjeF_N proteins, represented by mouse apolipoprotein A-I binding protein and the N-terminal domain of Tm0922, were found to interact with an adenosine diphosphoribose-related substrate and likely serve as ADP-ribosyltransferases. Crystallographic screening of metabolites serves as an efficient tool in functional analyses of uncharacterized proteins."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2012.07.016"xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2012.07.016"xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Joachimiak A."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Joachimiak A."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Lesley S.A."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Lesley S.A."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Minor W."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Minor W."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Shumilin I.A."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Shumilin I.A."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Cymborowski M."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Cymborowski M."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Chertihin O."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Chertihin O."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Herr J.C."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Herr J.C."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Jha K.N."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/author | "Jha K.N."xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/22940582 | http://purl.uniprot.org/core/name | "Structure"xsd:string |