| http://purl.uniprot.org/citations/2295629 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2295629 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2295629 | http://www.w3.org/2000/01/rdf-schema#comment | "A lectin was isolated from the homogenate of the tunicate Polyandrocarpa misakiensis by heat treatment, ammonium sulfate fractionation, gel filtration, and high-performance ion-exchange chromatography. Analytical gel filtration on Superose 12 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the lectin is a monomeric protein with a molecular mass of approximately 15 kDa. The lectin bound to an immobilized D-galactose column in the presence of calcium ion with a threshold of 500 microM and eluted completely with 5 mM EDTA. It did not bind to an immobilized D-mannose or N-acetyl-D-galactosamine column. Thus, Polyandrocarpa lectin was found to be a calcium-dependent galactose-binding lectin. The complete amino acid sequence of Polyandrocarpa lectin was determined by automated or manual Edman sequencing of the peptides derived by digestion with trypsin, endoproteinase Asp-N, Staphylococcus aureus V8 protease, and pepsin. It is composed of 125 residues, contains no carbohydrate group, and has a calculated molecular mass of 14,034 Da. The lectin contains four half-cystines, and Cys-21 and Cys-119 and also Cys-96 and Cys-111 form intrachain disulfide bridges, respectively. The amino acid sequence of Polyandrocarpa lectin shows about 20-30% homology with those of fly, barnacle, sea urchin, and several vertebrate lectins that belong to C-type lectin (Drickamer, K. (1988) J. Biol. Chem. 263, 9557-9560). Although the physiological role of Polyandrocarpa lectin is not clear, preliminary experiments suggest that the lectin may be related to defense mechanisms because it has a strong antibacterial activity."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)40009-4"xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)40009-4"xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Suzuki T."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Suzuki T."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Takagi T."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Takagi T."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Kawamura K."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Kawamura K."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Furukohri T."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Furukohri T."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Nakauchi M."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/author | "Nakauchi M."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/pages | "1274-1281"xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/pages | "1274-1281"xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/title | "A calcium-dependent galactose-binding lectin from the tunicate Polyandrocarpa misakiensis. Isolation, characterization, and amino acid sequence."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/title | "A calcium-dependent galactose-binding lectin from the tunicate Polyandrocarpa misakiensis. Isolation, characterization, and amino acid sequence."xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/volume | "265"xsd:string |
| http://purl.uniprot.org/citations/2295629 | http://purl.uniprot.org/core/volume | "265"xsd:string |