| http://purl.uniprot.org/citations/22972184 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22972184 | http://www.w3.org/2000/01/rdf-schema#comment | "Mouse embryonic stem (ES) cells consist of heterogeneous populations with differing abilities to proliferate and differentiate. We previously demonstrated that the expression level of platelet endothelial cell adhesion molecule 1 (PECAM1)/CD31 was positively correlated with the undifferentiated state of mouse ES cells. In order to screen for a novel gene(s) involved in ES cell pluripotency, we performed an oligo microarray analysis and identified that B-box and SPRY domain containing protein (BSPRY) was expressed at high levels in PECAM1-positive cells. Two splice isoforms of BSPRY, BSPRY-1 and BSPRY-2, were expressed in undifferentiated ES cells and in blastocysts. Knockdown of BSPRY-1/2 in ES cells significantly reduced the number of undifferentiated colonies and caused increased expression of primitive ectoderm marker gene Fgf5. The overexpression of BSPRY-2 reciprocally increased the number of undifferentiated ES cells in the presence of LIF. Similarly, injection of BSPRY-1/2 siRNAs into 2-cell embryos caused developmental retardation and degeneration of embryos, and a significant decrease in the number of cells, especially in the inner cell mass (ICM), was observed at the blastocyst stage. Furthermore, microinjection of a BSPRY-1 expression vector into pronuclear stage embryos resulted in an increase in the hatching blastocysts rate after 120 h of culture. These results suggest that BSPRY-1 and BSPRY-2 are associated with both ES cell pluripotency and early embryonic development."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.org/dc/terms/identifier | "doi:10.1262/jrd.2011-009"xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/author | "Ikeda M."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/author | "Furusawa T."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/author | "Tokunaga T."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/author | "Inoue F."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/author | "Ohkoshi K."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/author | "Tatemizo A."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/name | "J Reprod Dev"xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/pages | "691-699"xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/title | "B-box and SPRY domain containing protein (BSPRY) is associated with the maintenance of mouse embryonic stem cell pluripotency and early embryonic development."xsd:string |
| http://purl.uniprot.org/citations/22972184 | http://purl.uniprot.org/core/volume | "58"xsd:string |
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