| http://purl.uniprot.org/citations/22978324 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22978324 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22978324 | http://www.w3.org/2000/01/rdf-schema#comment | "Dystrobrevin family members (α and β) are cytoplasmic components of the dystrophin-associated glycoprotein complex, a multimeric protein complex first isolated from skeletal muscle, which links the extracellular matrix to the actin cytoskeleton. Dystrobrevin shares high homology with the cysteine-rich and C-terminal domains of dystrophin and a common domain organization. The β-dystrobrevin isoform is restricted to nonmuscle tissues, serves as a scaffold for signaling complexes, and may participate in intracellular transport through its interaction with kinesin heavy chain. We have previously characterized the molecular determinants affecting the β-dystrobrevin-kinesin heavy chain interaction, among which is cAMP-dependent protein kinase [protein kinase A (PKA)] phosphorylation of β-dystrobrevin. In this study, we have identified β-dystrobrevin residues phosphorylated in vitro by PKA with pull-down assays, surface plasmon resonance measurements, and MS analysis. Among the identified phosphorylated residues, we demonstrated, by site-directed mutagenesis, that Thr11 is the regulatory site for the β-dystrobrevin-kinesin interaction. As dystrobrevin may function as a signaling scaffold for kinases/phosphatases, we also investigated whether β-dystrobrevin is phosphorylated in vitro by kinases other than PKA. Thr11 was phosphorylated by protein kinase C, suggesting that this represents a key residue modified by the activation of different signaling pathways."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.org/dc/terms/identifier | "doi:10.1111/febs.12006"xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.org/dc/terms/identifier | "doi:10.1111/febs.12006"xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Macchia G."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Macchia G."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Crescenzi M."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Crescenzi M."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Fratini F."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Fratini F."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Salzano A.M."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Salzano A.M."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Matteucci A."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Matteucci A."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Petrucci T.C."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Petrucci T.C."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Macioce P."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Macioce P."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Ceccarini M."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Ceccarini M."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Torreri P."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/author | "Torreri P."xsd:string |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22978324 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |