| http://purl.uniprot.org/citations/22988298 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22988298 | http://www.w3.org/2000/01/rdf-schema#comment | "CrkRS (Cdc2-related kinase, Arg/Ser), or cyclin-dependent kinase 12 (CKD12), is a serine/threonine kinase believed to coordinate transcription and RNA splicing. While CDK12/CrkRS complexes were known to phosphorylate the C-terminal domain (CTD) of RNA polymerase II (RNA Pol II), the cyclin regulating this activity was not known. Using immunoprecipitation and mass spectrometry, we identified a 65-kDa isoform of cyclin K (cyclin K1) in endogenous CDK12/CrkRS protein complexes. We show that cyclin K1 complexes isolated from mammalian cells contain CDK12/CrkRS but do not contain CDK9, a presumed partner of cyclin K. Analysis of extensive RNA-Seq data shows that the 65-kDa cyclin K1 isoform is the predominantly expressed form across numerous tissue types. We also demonstrate that CDK12/CrkRS is dependent on cyclin K1 for its kinase activity and that small interfering RNA (siRNA) knockdown of CDK12/CrkRS or cyclin K1 has similar effects on the expression of a luciferase reporter gene. Our data suggest that cyclin K1 is the primary cyclin partner for CDK12/CrkRS and that cyclin K1 is required to activate CDK12/CrkRS to phosphorylate the CTD of RNA Pol II. These properties are consistent with a role of CDK12/CrkRS in regulating gene expression through phosphorylation of RNA Pol II."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.06267-11"xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Kuzyk M.A."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Marra M.A."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Ichu T.A."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Moradian A."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Morin G.B."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Tien J.F."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Griffith M."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Cheng S.W."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Chang V.C."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/author | "Vollett S.E."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/pages | "4691-4704"xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/title | "Interaction of cyclin-dependent kinase 12/CrkRS with cyclin K1 is required for the phosphorylation of the C-terminal domain of RNA polymerase II."xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/22988298 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22988298 |
| http://purl.uniprot.org/citations/22988298 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22988298 |
| http://purl.uniprot.org/uniprot/O75909#attribution-296529BE82488EFF2E1529D05FF9EB47 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22988298 |
| http://purl.uniprot.org/uniprot/O75909#attribution-5A716B851BE90C489E5E223B87569A06 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22988298 |
| http://purl.uniprot.org/uniprot/Q9NYV4#attribution-296529BE82488EFF2E1529D05FF9EB47 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22988298 |
| http://purl.uniprot.org/uniprot/Q9NYV4#attribution-5A716B851BE90C489E5E223B87569A06 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22988298 |
| http://purl.uniprot.org/uniprot/#_P13984-mappedCitation-22988298 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22988298 |