| http://purl.uniprot.org/citations/22991218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22991218 | http://www.w3.org/2000/01/rdf-schema#comment | "LASS2/TMSG1 was a novel tumor metastasis suppressor gene, which was first cloned by our laboratory from non-metastatic and metastatic cancer cell variants of human prostate carcinoma PC-3M using mRNA differential display in 1999. LASS2/TMSG1 could interact with the C subunit of vacuolar ATPase (V-ATPase, ATP6V0C) and regulate V-ATPase activity. In an attempt to provide molecular mechanism of the interaction between LASS2/TMSG1 and V-ATPase, we constructed four variant transfectants containing different functional domain of LASS2/TMSG1 and stably transfected the variants to human prostate cancer cell line PC-3M-1E8 cell with high metastatic potential. Results showed that there were no obvious differences of V-ATPase expression among different transfected cells and the control. However, V-ATPase activity and intracellular pH was significantly higher in the variant transfectants with Homeodomain of LASS2/TMSG1 than that in the control using the pH-dependent fluorescence probe BECEF/AM. Immunoprecipitation, immunofluorescence and immuno-electron microscope alone or in combination demonstrated the direct interaction of Homeodomain of LASS2/TMSG1 and ATP6V0C. Loss of Homeodomain markedly enhanced the proliferation ability but weakened the apoptotic effect of LASS2/TMSG1 in PC-3M-1E8 cells. These lines of results for the first time contribute to the conclusion that LASS2/TMSG1 could regulate V-ATPase activity and intracellular pH through the direct interaction of its Homeodomain and the C subunit of V-ATPase. Their interaction could play important roles in the apoptosis of tumor cells."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.24400"xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Gong M."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Xu X."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Zheng J."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Yu W."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "You J."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Zou P."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Pei F."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/author | "Mei F."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/name | "J Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/pages | "570-583"xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/title | "A novel tumor metastasis suppressor gene LASS2/TMSG1 interacts with vacuolar ATPase through its homeodomain."xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://purl.uniprot.org/core/volume | "114"xsd:string |
| http://purl.uniprot.org/citations/22991218 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22991218 |
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