| http://purl.uniprot.org/citations/22992742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22992742 | http://www.w3.org/2000/01/rdf-schema#comment | "We performed a proteomics screen for Rho isoform-specific binding proteins to clarify the tumor-promoting effects of RhoA and C that contrast with the tumor-suppressive effects of RhoB. We found that the IQ-motif-containing GTPase-activating protein IQGAP1 interacts directly with GTP-bound, prenylated RhoA and RhoC, but not with RhoB. Co-immunoprecipitation of IQGAP1 with endogenous RhoA/C was enhanced when RhoA/C were activated by epidermal growth factor (EGF) or transfection of a constitutively active guanine nucleotide exchange factor (GEF). Overexpression of IQGAP1 increased GTP-loading of RhoA/C, while siRNA-mediated depletion of IQGAP1 prevented endogenous RhoA/C activation by growth factors. IQGAP1 knockdown also reduced the amount of GTP bound to GTPase-deficient RhoA/C mutants, suggesting that IQGAP enhances Rho activation by GEF(s) or stabilizes Rho-GTP. IQGAP1 depletion in MDA-MB-231 breast cancer cells blocked EGF- and RhoA-induced stimulation of DNA synthesis. Infecting cells with adenovirus encoding constitutively active RhoA(L63) and measuring absolute amounts of RhoA-GTP in infected cells demonstrated that the lack of RhoA(L63)-induced DNA synthesis in IQGAP1-depleted cells was not due to reduced GTP-bound RhoA. These data suggested that IQGAP1 functions downstream of RhoA. Overexpression of IQGAP1 in MDA-MB-231 cells increased DNA synthesis irrespective of siRNA-mediated RhoA knockdown. Breast cancer cell motility was increased by expressing a constitutively-active RhoC(V14) mutant or overexpressing IQGAP1. EGF- or RhoC-induced migration required IQGAP1, but IQGAP1-stimulated migration independently of RhoC, placing IQGAP1 downstream of RhoC. We conclude that IQGAP1 acts both upstream of RhoA/C, regulating their activation state, and downstream of RhoA/C, mediating their effects on breast cancer cell proliferation and migration, respectively."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m112.377499"xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/author | "Turner S."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/author | "Zhuang S."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/author | "Boss G.R."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/author | "Casteel D.E."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/author | "Pilz R.B."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/author | "Schwappacher R."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/author | "Rangaswami H."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/author | "Su-Yuo J."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/pages | "38367-38378"xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/title | "Rho isoform-specific interaction with IQGAP1 promotes breast cancer cell proliferation and migration."xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://purl.uniprot.org/core/volume | "287"xsd:string |
| http://purl.uniprot.org/citations/22992742 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22992742 |
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