| http://purl.uniprot.org/citations/22996741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22996741 | http://www.w3.org/2000/01/rdf-schema#comment | "MicroRNA-155 (miR-155) overexpression is often found in malignancies including lung cancer. The objective of this study is to verify the hypothesis, based on the results of bioinformatics analysis, that miR-155 modulates cellular apoptosis and DNA damage through the regulation of Apaf-1 and is thus involved in the development and progression of lung cancer. First, we measured the expression of miR-155 and the Apaf-1 protein in lung cancer tissues. The results showed that expression of miR-155 was significantly higher in lung cancer tissues than in paracancerous and normal tissues; whereas Apaf-1 expression was lower in the lung cancerous tissues. We then established miR-155-silenced and Apaf-1-overexpressed A549 cell lines by transfection with pMAGic2.0-BIC-siRNA and pcDNA3.1-Apaf-1, respectively. These cell lines were then treated with cisplatin, and apoptosis and DNA damage were assessed, with non-transfected A549 cells used as negative controls. The results showed that, relative to controls, the silencing of miR-155 resulted in elevated expression of the Apaf-1 protein, whereas Apaf-1 mRNA levels remained unchanged. Both the silencing of miR-155 and the overexpression Apaf-1 greatly increased the sensitivity of A549 cells to cisplatin treatment, as evidenced by elevated rates of apoptosis and DNA damage. Furthermore, dual-transfection of A549 cells with miR-155 siRNA and Apaf-1 siRNA resulted in the attenuation of apoptosis and DNA damage. In conclusion, the inhibition of miR-155 can enhance the sensitivity of A549 cells to cisplatin treatment by modulation of cellular apoptosis and DNA damage through an Apaf-1-mediated pathway."xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.org/dc/terms/identifier | "doi:10.1038/cgt.2012.60"xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/author | "An J."xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/author | "Li B."xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/author | "Fang Z."xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/author | "Zhong Y.F."xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/author | "Zang Y.S."xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/name | "Cancer Gene Ther"xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/pages | "773-778"xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/title | "MiR-155 inhibits the sensitivity of lung cancer cells to cisplatin via negative regulation of Apaf-1 expression."xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/22996741 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22996741 |
| http://purl.uniprot.org/citations/22996741 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22996741 |
| http://purl.uniprot.org/uniprot/#_A7E2A2-mappedCitation-22996741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22996741 |
| http://purl.uniprot.org/uniprot/#_A5YM44-mappedCitation-22996741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22996741 |
| http://purl.uniprot.org/uniprot/#_O14727-mappedCitation-22996741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22996741 |
| http://purl.uniprot.org/uniprot/O14727 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22996741 |
| http://purl.uniprot.org/uniprot/A5YM44 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22996741 |
| http://purl.uniprot.org/uniprot/A7E2A2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22996741 |