| http://purl.uniprot.org/citations/23000411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23000411 | http://www.w3.org/2000/01/rdf-schema#comment | "High molecular weight kininogen (HK) is a plasma glycoprotein with multiple functions, including the regulation of coagulation. We previously demonstrated that domain 5 (D5(H)), a functional domain of HK, and its derived peptides played an important role in the vitronectin-mediated suppression of cancer cell adhesion and invasion. However, the underlying mechanisms of the D5(H)-mediated suppressive effects remain to be elucidated. Here, we showed that D5(H) and its derivatives inhibited the collagen-mediated cell adhesion and invasion of human osteosarcoma MG63 cells. Using purified D5(H) fused to glutathione-S-transferase (GST) and D5(H)-derived peptides for column chromatography, an actin-binding protein, α-actinin-4, was identified as a binding protein of D5(H) with high-affinity for P-5m, a core octapeptide of D5(H). Immunofluorescence microscopy demonstrated that D5(H) co-localized with α-actinin-4 inside MG63 cells. In addition, exogenous GST-D5(H) added to the culture media was transported into MG63 cells, although GST alone as a control was not. As α-actinin-4 regulates actin polymerization necessary for cell adhesion and is related to the integrin-dependent attachment of cells to the extracellular matrix, our results suggest that D5(H) may modulate cell adhesion and invasion together with actinin-4."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2012.09.079"xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/author | "Takeuchi K."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/author | "Uchiyama S."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/author | "Maeda T."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/author | "Otsuka T."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/author | "Ogita H."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/author | "Ohkubo I."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/author | "Ogikubo O."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/author | "Hatoh T."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/pages | "497-502"xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/title | "Domain 5 of high molecular weight kininogen inhibits collagen-mediated cancer cell adhesion and invasion in association with alpha-actinin-4."xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://purl.uniprot.org/core/volume | "427"xsd:string |
| http://purl.uniprot.org/citations/23000411 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23000411 |
| http://purl.uniprot.org/citations/23000411 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23000411 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z3X3-mappedCitation-23000411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23000411 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z3C0-mappedCitation-23000411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23000411 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z3G1-mappedCitation-23000411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23000411 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z3G9-mappedCitation-23000411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23000411 |
| http://purl.uniprot.org/uniprot/#_B4E1C2-mappedCitation-23000411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23000411 |
| http://purl.uniprot.org/uniprot/#_B4DPP8-mappedCitation-23000411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23000411 |
| http://purl.uniprot.org/uniprot/#_B4E337-mappedCitation-23000411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23000411 |