| http://purl.uniprot.org/citations/23017427 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23017427 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23017427 | http://www.w3.org/2000/01/rdf-schema#comment | "The 155-kDa plasma glycoprotein factor H (FH), which consists of 20 complement control protein (CCP) modules, protects self-tissue but not foreign organisms from damage by the complement cascade. Protection is achieved by selective engagement of FH, via CCPs 1-4, CCPs 6-8 and CCPs 19-20, with polyanion-rich host surfaces that bear covalently attached, activation-specific, fragments of complement component C3. The role of intervening CCPs 9-18 in this process is obscured by lack of structural knowledge. We have concatenated new high-resolution solution structures of overlapping recombinant CCP pairs, 10-11 and 11-12, to form a three-dimensional structure of CCPs 10-12 and validated it by small-angle X-ray scattering of the recombinant triple-module fragment. Superimposing CCP 12 of this 10-12 structure with CCP 12 from the previously solved CCP 12-13 structure yielded an S-shaped structure for CCPs 10-13 in which modules are tilted by 80-110° with respect to immediate neighbors, but the bend between CCPs 10 and 11 is counter to the arc traced by CCPs 11-13. Including this four-CCP structure in interpretation of scattering data for the longer recombinant segments, CCPs 10-15 and 8-15, implied flexible attachment of CCPs 8 and 9 to CCP 10 but compact and intimate arrangements of CCP 14 with CCPs 12, 13 and 15. Taken together with difficulties in recombinant production of module pairs 13-14 and 14-15, the aberrant structure of CCP 13 and the variability of 13-14 linker sequences among orthologues, a structural dependency of CCP 14 on its neighbors is suggested; this has implications for the FH mechanism."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2012.09.013"xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2012.09.013"xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Soares D.C."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Soares D.C."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Svergun D.I."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Svergun D.I."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Barlow P.N."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Barlow P.N."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Schmidt C.Q."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Schmidt C.Q."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Herbert A.P."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Herbert A.P."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Mertens H.D."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Mertens H.D."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Maciejewski M."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Maciejewski M."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Makou E."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Makou E."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Matis I."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/author | "Matis I."xsd:string |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23017427 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |