DESCRIBE <http://purl.uniprot.org/citations/23029280>

Subject	Predicate	Object
http://purl.uniprot.org/citations/23029280	http://www.w3.org/1999/02/22-rdf-syntax-ns#type	http://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23029280	http://www.w3.org/2000/01/rdf-schema#comment	" Background Drugs inhibiting vascular endothelial growth factor (VEGF) signaling are globally administered to suppress deregulated angiogenesis in a variety of eye diseases. However, anti-VEGF therapy potentially affects the normal functions of retinal neurons and glias which constitutively express VEGF receptor 2. Thus, it is desirable to identify novel drug targets which are exclusively expressed in endothelial cells (ECs). Here we attempted to identify an EC-specific Rho guanine nucleotide exchange factor (GEF) and evaluate its role in retinal angiogenesis. Methodology/principal findings By exploiting fluorescence-activated cell sorting and microarray analyses in conjunction with in silico bioinformatics analyses, we comprehensively identified endothelial genes in angiogenic retinal vessels of postnatal mice. Of 9 RhoGEFs which were highly expressed in retinal ECs, we show that Arhgef15 acted as an EC-specific GEF to mediate VEGF-induced Cdc42 activation and potentiated RhoJ inactivation, thereby promoting actin polymerization and cell motility. Disruption of the Arhgef15 gene led to delayed extension of vascular networks and subsequent reduction of total vessel areas in postnatal mouse retinas. Conclusions/significance Our study provides information useful to the development of new means of selectively manipulating angiogenesis without affecting homeostasis in un-targeted tissues; not only in eyes but also in various disease settings such as cancer."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.org/dc/terms/identifier	"doi:10.1371/journal.pone.0045858"xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Nishida K."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Nishikawa S."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Okada M."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Shimizu Y."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Mochizuki N."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Uemura A."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Fukuhara S."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Fukushima Y."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Hata M."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Jakt L.M."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Hirashima M."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Kusuhara S."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/author	"Negi A."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/date	"2012"xsd:gYear
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/name	"PLoS One"xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/pages	"e45858"xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/title	"Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells."xsd:string
http://purl.uniprot.org/citations/23029280	http://purl.uniprot.org/core/volume	"7"xsd:string
http://purl.uniprot.org/citations/23029280	http://www.w3.org/2004/02/skos/core#exactMatch	http://purl.uniprot.org/pubmed/23029280
http://purl.uniprot.org/citations/23029280	http://xmlns.com/foaf/0.1/primaryTopicOf	https://pubmed.ncbi.nlm.nih.gov/23029280
http://purl.uniprot.org/uniprot/#_A0A0S2Z543-mappedCitation-23029280	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/23029280
http://purl.uniprot.org/uniprot/#_A0A0S2Z547-mappedCitation-23029280	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/23029280