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http://purl.uniprot.org/citations/23043599http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23043599http://www.w3.org/2000/01/rdf-schema#comment"

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The influenza A virus (IAV) protein PB1-F2, which is encoded by an alternative ORF of the PB1 polymerase gene, has been implicated as an important virulence factor and apoptosis inducer. However, the molecular mechanism of PB1-F2 function remains elusive. In this study, eight cellular proteins were identified as potential PB1-F2 interacting partners using the yeast two-hybrid system. Two positive candidate proteins, guanine nucleotide binding protein (G protein) beta polypeptide 2 (Gβ2) and macrophage migration inhibitory factor (MIF), were selected to be further characterized. The interaction of MIF and Gβ2 with PB1-F2 was confirmed by both GST pull-down and co-immunoprecipitation assays. Confocal laser microscopy showed that the interaction between PB1-F2 and the two cellular proteins occurred in the cytoplasm. The novel interactions between PB1-F2 and host proteins provide further pieces of evidence in the investigation of the pathogenic mechanism of IAV.

Keywords

influenza A virus; PB1-F2; yeast two-hybrid; protein-protein interaction."xsd:string
http://purl.uniprot.org/citations/23043599http://purl.org/dc/terms/identifier"doi:10.4149/av_2012_03_199"xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/author"Zhang M.L."xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/author"Wang X.R."xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/author"Duan M."xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/author"Cui Y.M."xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/author"Guan Z.H."xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/author"Hou P.L."xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/name"Acta Virol"xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/pages"199-207"xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/title"Identification of cellular proteins interacting with influenza A virus PB1-F2 protein."xsd:string
http://purl.uniprot.org/citations/23043599http://purl.uniprot.org/core/volume"56"xsd:string
http://purl.uniprot.org/citations/23043599http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23043599
http://purl.uniprot.org/citations/23043599http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/23043599
http://purl.uniprot.org/uniprot/#_P14174-mappedCitation-23043599http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23043599
http://purl.uniprot.org/uniprot/#_I4AY87-mappedCitation-23043599http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23043599
http://purl.uniprot.org/uniprot/#_P62879-mappedCitation-23043599http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23043599
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http://purl.uniprot.org/uniprot/P14174http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23043599
http://purl.uniprot.org/uniprot/P62879http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23043599
http://purl.uniprot.org/uniprot/Q6FHM2http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23043599
http://purl.uniprot.org/uniprot/I4AY87http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23043599