| http://purl.uniprot.org/citations/23050238 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23050238 | http://www.w3.org/2000/01/rdf-schema#comment | "Systematic analysis of gene overexpression phenotypes provides an insight into gene function, enzyme targets, and biological pathways. Here, we describe a novel functional genomics platform that enables a highly parallel and systematic assessment of overexpression phenotypes in pooled cultures. First, we constructed a genome-level collection of ~5100 yeast barcoder strains, each of which carries a unique barcode, enabling pooled fitness assays with a barcode microarray or sequencing readout. Second, we constructed a yeast open reading frame (ORF) galactose-induced overexpression array by generating a genome-wide set of yeast transformants, each of which carries an individual plasmid-born and sequence-verified ORF derived from the Saccharomyces cerevisiae full-length EXpression-ready (FLEX) collection. We combined these collections genetically using synthetic genetic array methodology, generating ~5100 strains, each of which is barcoded and overexpresses a specific ORF, a set we termed "barFLEX." Additional synthetic genetic array allows the barFLEX collection to be moved into different genetic backgrounds. As a proof-of-principle, we describe the properties of the barFLEX overexpression collection and its application in synthetic dosage lethality studies under different environmental conditions."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.org/dc/terms/identifier | "doi:10.1534/g3.112.003400"xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Smith A.M."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Yan Z."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Nislow C."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Lee A.Y."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Giaever G."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Boone C."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Andrews B.J."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "van Dyk D."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Ryan O."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Durbic T."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Sharifpoor S."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Heisler L.E."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Gottert H."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Douglas A.C."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/author | "Surendra A."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/name | "G3 (Bethesda)"xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/pages | "1279-1289"xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/title | "Functional analysis with a barcoder yeast gene overexpression system."xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://purl.uniprot.org/core/volume | "2"xsd:string |
| http://purl.uniprot.org/citations/23050238 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23050238 |
| http://purl.uniprot.org/citations/23050238 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23050238 |