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http://purl.uniprot.org/citations/23064328http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23064328http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23064328http://www.w3.org/2000/01/rdf-schema#comment"Viral production estimates show that virioplankton communities turn over rapidly in aquatic ecosystems. Thus, it is likely that the genetic identity of viral populations comprising the virioplankton also change over temporal and spatial scales, reflecting shifts in viral-host interactions. However, there are few approaches that can provide data on the genotypic identity of viral populations at low cost and with the sample throughput necessary to assess dynamic changes in the virioplankton. This study examined two of these approaches-T4-like major capsid protein (g23) gene polymorphism and randomly amplified polymorphic DNA-PCR (RAPD-PCR) fingerprinting-to ask how well each technique could track differences in virioplankton populations over time and geographic location. Seasonal changes in overall virioplankton composition were apparent from pulsed-field gel electrophoresis (PFGE) analysis. T4-like phages containing similar g23 proteins were found within both small- and large-genome populations, including populations from different geographic locations and times. The surprising occurrence of T4-like g23 within small genomic groups (23 to 64 kb) indicated that the genome size range of T4-like phages may be broader than previously believed. In contrast, RAPD-PCR fingerprinting detected high genotypic similarity within PFGE bands from the same location, time, and genome size class without the requirement for DNA sequencing. Unlike g23 polymorphism, RAPD-PCR fingerprints showed a greater temporal than geographic variation. Thus, while polymorphism in a viral signature gene, such as g23, can be a powerful tool for inferring evolutionary relationships, the degree to which this approach can capture fine-scale variability within virioplankton populations is less clear."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.org/dc/terms/identifier"doi:10.1128/AEM.02432-12"xsd:string
http://purl.uniprot.org/citations/23064328http://purl.org/dc/terms/identifier"doi:10.1128/aem.02432-12"xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Polson S.W."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Polson S.W."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Wommack K.E."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Wommack K.E."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Jamindar S."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Jamindar S."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Srinivasiah S."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Srinivasiah S."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Waidner L."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/author"Waidner L."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/name"Appl. Environ. Microbiol."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/name"Appl Environ Microbiol"xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/pages"8773-8783"xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/pages"8773-8783"xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/title"Evaluation of two approaches for assessing the genetic similarity of virioplankton populations as defined by genome size."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/title"Evaluation of two approaches for assessing the genetic similarity of virioplankton populations as defined by genome size."xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/volume"78"xsd:string
http://purl.uniprot.org/citations/23064328http://purl.uniprot.org/core/volume"78"xsd:string
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