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http://purl.uniprot.org/citations/23065795http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23065795http://www.w3.org/2000/01/rdf-schema#comment"

Scope

This study further examines mechanisms involved in the pro-apoptotic action of gamma-tocopherol (γT) and gamma-tocotrienol (γT3) in human breast cancer cell lines.

Methods and results

γT upregulates phospho-JNK (pJNK), CCAAT/enhancer-binding protein homologous protein (CHOP), and death receptor-5 (DR5) protein expression as detected by Western blot assays. siRNA knockdown of JNK, CHOP, or DR5 shows that γT-induced apoptosis is JNK/CHOP/DR5 signaling dependent, which is similar to γT3-mediated apoptotic signaling. Furthermore, both γT and γT3 induce increased levels of cellular ceramides and dihydroceramides as determined by LC-MS/MS analyses. Inhibition of de novo ceramide synthesis using chemical inhibitors blocked the ability of γT and γT3 to induce apoptosis as detected by Annexin V-FITC/PI assay and to activate JNK/CHOP/DR5 pro-apoptotic signaling thereby demonstrating the involvement of de novo ceramide synthesis in γT- and γT3-induced apoptosis.

Conclusion

Taken together, data show that both γT and γT3 induce apoptosis via de novo ceramide synthesis dependent activation of JNK/CHOP/DR5 pro-apoptotic signaling."xsd:string
http://purl.uniprot.org/citations/23065795http://purl.org/dc/terms/identifier"doi:10.1002/mnfr.201200350"xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/author"Jiang Q."xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/author"Yu W."xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/author"Jang Y."xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/author"Gopalan A."xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/author"Kline K."xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/author"Sanders B.G."xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/name"Mol Nutr Food Res"xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/pages"1803-1811"xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/title"Involvement of de novo ceramide synthesis in gamma-tocopherol and gamma-tocotrienol-induced apoptosis in human breast cancer cells."xsd:string
http://purl.uniprot.org/citations/23065795http://purl.uniprot.org/core/volume"56"xsd:string
http://purl.uniprot.org/citations/23065795http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23065795
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