| http://purl.uniprot.org/citations/23085076 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23085076 | http://www.w3.org/2000/01/rdf-schema#comment | "RCK domains control activity of a variety of K(+) channels and transporters through binding of cytoplasmic ligands. To gain insight toward mechanisms of RCK domain activation, we solved the structure of the RCK domain from the Ca(2+)-gated K(+) channel, MthK, bound with Ba(2+), at 3.1 Å resolution. The Ba(2+)-bound RCK domain was assembled as an octameric gating ring, as observed in structures of the full-length MthK channel, and shows Ba(2+) bound at several positions. One of the Ba(2+) sites, termed C1, overlaps with a known Ca(2+)-activation site, determined by residues D184 and E210. Functionally, Ba(2+) can activate reconstituted MthK channels as observed in electrophysiological recordings, whereas Mg(2+) (up to 100 mM) was ineffective. Ba(2+) activation was abolished by the mutation D184N, suggesting that Ba(2+) activates primarily through the C1 site. Our results suggest a working hypothesis for a sequence of ligand-dependent conformational changes that may underlie RCK domain activation and channel gating."xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2012.09.014"xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/author | "Cingolani G."xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/author | "Rothberg B.S."xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/author | "Smith F.J."xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/author | "Pau V.P."xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/pages | "2038-2047"xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/title | "Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation."xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/23085076 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23085076 |
| http://purl.uniprot.org/citations/23085076 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23085076 |
| http://purl.uniprot.org/uniprot/O27564#attribution-0D3146D0587FE780D84220D6D261A184 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23085076 |
| http://purl.uniprot.org/uniprot/#_O27564-mappedCitation-23085076 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23085076 |
| http://purl.uniprot.org/uniprot/O27564 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23085076 |